Ufa, Russian Federation
employee
Ufa, Ufa, Russian Federation
Ufa, Ufa, Russian Federation
employee
Ufa, Ufa, Russian Federation
from 01.01.2018 until now
Ufa, Ufa, Russian Federation
UDK 616.31 Стоматология. Заболевания ротовой полости и зубов
Subject. Many researchers emphasize that most congenital defects have a multifactorial nature and, along with a number of other diseases (neoplasms, bronchial asthma, etc.), can serve as indicators of an unfavorable state of the environment. Among the key targets of the negative impact of environmental and industrial factors in the human body, the DNA molecule is distinguished. Objective. To analyze the severity of DNA integrity disorders (fragmentation) in children with congenital cleft lip and palate from regions with industrial petrochemical ecotoxicants using gel electrophoresis of single leukocytes. Methodology. A total of 60 children with congenital cleft lip and palate from regions with industrial petrochemical ecotoxicants were selected, as well as 40 children without the corresponding pathology from the same regions included in the control group. The level of DNA damage was assessed on peripheral blood lymphocytes of donors aged 5–12 years. The degree of DNA damage in single leukocytes of venous blood was determined by the alkaline version of the DNA comet assay by performing alkaline gel electrophoresis of individual cells. Results. The analysis of the obtained data indicates a high level of genotoxic stress in children with congenital cleft lip and palate (CLPP), which differs significantly from similar indicators in the control group. Reliable differences in a number of key parameters (tail length, percentage of DNA in the tail, tail moment) indicate more intense processes of fragmentation of deoxyribonucleic acid in patients with congenital anomalies. Taken together, these results suggest that this cohort has an increased sensitivity to adverse environmental factors, in particular, to toxic and mutagenic substances present in their places of permanent residence. Conclusions. The obtained data are a new aspect in the pathogenesis of congenital cleft lip and palate and can be used to predict this defect in children living in a region with industrial petrochemical ecotoxicants.
congenital cleft lip, congenital cleft palate, congenital cleft lip and palate, region with ecotoxicants, DNA comet assay, DNA damage, forecasting
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