employee
Ufa, Russian Federation
Ufa, Ufa, Russian Federation
graduate student from 01.01.2018 until now
Ufa, Ufa, Russian Federation
employee
Ufa, Ufa, Russian Federation
Ufa, Russian Federation
employee from 01.01.1983 until now
Ufa, Ufa, Russian Federation
employee from 01.01.2004 until now
Ufa, Ufa, Russian Federation
UDK 616.31 Стоматология. Заболевания ротовой полости и зубов
Objectives. Immunological, biochemical and physicochemical analysis of oral fluid in children with congenital cleft palate. Purpose. To analyze oral fluid in children with congenital cleft palate to determine the levels of cytokines (IL-2, IL-4, IL-6), lysozyme, secretory immunoglobulin A, interferon gamma and evaluate biochemical (calcium, magnesium, phosphorus, protein) and physicochemical (salivation rate, kinematic viscosity, pH) parameters of mixed saliva. Methodology. The article presents the results of a laboratory study of the parameters of the oral fluid of 90 children aged 2–5 years with congenital cleft and 45 practically healthy children of the same age. Results. In children 2–5 years old with congenital cleft palate, according to immunological parameters of the oral fluid, there is a decrease in the amount of cytokines in the oral fluid (IL-2, IL-4, IL-6), lysozyme, secretory immunoglobulin-A, interferon gamma, which indicates deficiencies of local immunity in the oral cavity and low resistance to bacterial infections. An increase in the kinematic viscosity of the oral fluid and a decrease in protein levels were noted. Conclusions. Laboratory confirmation was obtained of the presence of a local inflammatory process in the oral cavity and a decrease in local immunological anti-inflammatory factors in the oral fluid. Changes in the immunological and rheological properties of oral fluid in children with congenital cleft palate lead to disturbances in the process of self-cleaning of the mucous membrane and teeth, which in the postoperative period creates high risks for the formation of infection and inflammation of the wound after uranoplasty.
congenital cleft palate, immunological parameters of oral fluid, cytokines, s-IgA, biochemistry of oral fluid
1. Mitropanova M.N. Osobennosti funkcionirovaniya immunnoy sistemy u detey s vrozhdennymi rasschelinami guby i neba na etapah hirurgicheskogo lecheniya. Stomatologiya detskogo vozrasta i profilaktika. 2017;16(61):79-83. [M.N. Mitropanova. Features of the functioning of the immune system in children with congenital cleft lip and palate at the stages of surgical treatment. Dentistry of childhood and prevention. 2017;16(61):79-83. (In Russ.)]. https://elibrary.ru/item.asp?id=29206060
2. Nesterova I.V., Mitropanova M.N., Chudilova G.A., Lomtatidze L.V., Gayvoronskaya T.V. Vliyanie disbalansa regulyatornyh citokinov i osteokal'cina na osteogenez u detey s vrozhdennoy rasschelinoy guby i neba v postnatal'nom ontogeneze. Stomatologiya. 2020;99(1):77-81. [I.V. Nesterova, M.N. Mitropanova, G.A. Chudilova, L.V. Lomtatidze, T.V. Gaivoronskaya. Effect of imbalance of regulatory cytokines and osteocalcin on osteogenesis in children with congenital cleft lip and palate in postnatal ontogenesis. Dentistry. 2020;99(1):77-81. (In Russ.)]. DOI:https://doi.org/10.17116/stomat20209901177
3. Rogova L.N., Fomenko I.V., Timoshenko A.N. Immunologicheskaya i mikrobiologicheskaya harakteristika slizistoy obolochki polosti rta u detey s vrozhdennoy rasschelinoy verhney guby i neba (obzor literatury). Volgogradskiy nauchno-medicinskiy zhurnal. 2016;3(51):19-22. [L.N. Rogova, I.V. Fomenko, A.N. Timoshenko. Immunological and microbiological characteristics of the oral mucosa in children with congenital cleft lip and palate (literature review). Volgograd Scientific Medical Journal. 2016;3(51):19-22. (In Russ.)]. https://elibrary.ru/item.asp?id=27249177
4. Hripach L.V., Knyazeva T.D., Zheleznyak E.V., Makoveckaya A.K., Koganova Z.I., Budarina O.V., Lebedeva N.V., Ingel' F.I., Demina N.N. Adaptivnye izmeneniya biohimicheskih i immunologicheskih pokazateley smeshannoy slyuny pri vozdeystvii zagryazneniy atmosfernogo vozduha na detey doshkol'nogo vozrasta. Mezhdunarodnyy zhurnal prikladnyh i fundamental'nyh issledovaniy. 2019;6:68-73. [L.V. Khripach, T.D. Knyazeva, E.V. Zheleznyak, A.K. Makovetskaya, Z.I. Koganova, O.V. Budarina, N.V. Lebedeva, F.I. Ingel, N.N. Demina. Adaptive changes in biochemical and immunological parameters of mixed saliva under the influence of atmospheric air pollution on preschool children. International Journal of Applied and Basic Research. 2019;6:68-73. (In Russ.)]. https://elibrary.ru/item.asp?id=38489006
5. Osokina A.S., Maslak E.E., Yakovlev A.T. Uroven' immunoglobulina A v slyune v zavisimosti ot nalichiya i tyazhesti rannego detskogo kariesa. Stomatologiya detskogo vozrasta i profilaktika. 2020;20;4(76):304-309. [A.S. Osokina, E.E. Maslak, A.T. Yakovlev. The level of immunoglobulin A in saliva depending on the presence and severity of early childhood caries. Dentistry of childhood and prevention. 2020;20;4(76):304-309. (In Russ.)]. DOI:https://doi.org/10.33925/1683-3031-2020-20-4-304-309
6. Danilova M.A., Aleksandrova L.I. Kachestvo zhizni detey s vrozhdennoy rasschelinoy guby i neba. Stomatologiya detskogo vozrasta i profilaktika. 2018;17(3):54-57. [M.A. Danilova, L.I. Aleksandrova. Quality of life in children with congenital cleft lip and palate. Dentistry of childhood and prevention. 2018;17(3):54-57. (In Russ.)]. DOI:https://doi.org/10.25636/PMP.3.2018.3.10
7. Shevchenko O.L., Antonova A.A. Sostav smeshannoy slyuny i pokazateli kariesa vremennyh zubov i ego oslozhneniy u detey. Endodontiya Today. 2015;4:8-11. [O.L. Shevchenko, A.A. Antonova. The composition of mixed saliva and indicators of caries in deciduous teeth and its complications in children. Endodontics Today. 2015;4:8-11. (In Russ.)]. https://elibrary.ru/item.asp?id=25411139
8. Musahodzhaeva D.A., Inoyatov A.Sh., Yakubov Sh.N. Nekotorye pokazateli immunnoy sistemy detey s vrozhdennoy rasschelinoy guby i neba. Problemy biologii i mediciny. 2011;4(67):33. [D.A. Musakhodzhaeva, A.Sh. Inoyatov, Sh.N. Yakubov. Some indicators of the immune system of children with congenital cleft lip and palate. Problems of biology and medicine. 2011;4(67):33. (In Russ.)]. https://inlibrary.uz/index.php/problems_biology/article/view/6728
9. Agaeva N.A. Rol' sekretornogo IgA v patologii chelyustno-licevoy oblasti. Fundamental'nye issledovaniya. 2010;4:11-16. [N.A. Agayeva. The role of secretory IgA in the pathology of the maxillofacial region. Basic research. 2010;4:11-16. (In Russ.)]. https://elibrary.ru/item.asp?id=15107695
10. Chuykin O.S., Kuchuk K.N., Chuykin S.V., Mochalov K.S., Davletshin N.A., Akat'eva G.G., Makusheva N.V., Ganieva R.A. Immunologicheskie, fiziko-himicheskie i biohimicheskie pokazateli rotovoy zhidkosti u detey s vrozhdennoy rasschelinoy neba i posleoperacionnym defektom neba. Problemy stomatologii. 2022;18(1):121-129. [O.S. Chuikin, K.N. Kuchuk, S.V. Chuikin, K.S. Mochalov, N.A. Davletshin, G.G. Akatieva, N.V. Makusheva, R.A. Ganieva. Immunological, physicochemical and biochemical parameters of oral fluid in children with congenital cleft palate and postoperative palate defect. Actual problems of dentistry. 2022;18(1):121-129. (In Russ.)]. DOIhttps://doi.org/10.18481/2077-7566-22-18-1-121-129.
