employee
Ekaterinburg, Ekaterinburg, Russian Federation
UDK 616 Патология. Клиническая медицина
Subject. The subject of this research is the immunological parameters of the blood serum and the oral fluid. Objectives. The objective of this research is to study the levels of serum and oral cytokines TNF-α, IFN-γ, IL-4, MCP-1 in women with secondary amenorrhea for at least one year. Methodology. There were 109 women under the supervision of the Ural State Medical University Department of Preventive Dentistry and Propedeutics of Dental Disease. The main group consisted of 76 women with generalized moderate chronic periodontitis (K05.31)and secondary amenorrhea for at least one year. The control group consisted of 33 post-menopausal women with generalized slight chronic periodontitis in remission. The patients of the both groups underwent laboratory serum and oral fluid testing using enzyme-linked immunosorbent assay. The levels of cytokines TNF-α, IFN-γ, IL-4, MCP-1 were measured in samples using Vector-Best’s tests systems (Vector-Best, Koltsovo, Novosibirsk Region, Russia). The systemic bone mineral density was measured using dual energy X-ray absorptiometry (DXA) with Discovery W QDR Series X-Ray Bone Densitometer (Hologic Inc., USA). Conclusion. The levels of interleikins TNF-α, IFN-γ and chemokine MCP-1 were found to be elevated in the blood serum and the oral fluid of women generalized moderate chronic periodontitis against the background of secondary amenorrhea for at least one year. In contrast, the serum values of IL-4 revealed statistically lower levels in comparison with the same values of IL-4 in the patients from the control group. We defined the statistically significant (at 0.05) correlation between parameters of salivary IL-4 levels and spine bone mineral density, and also the statistically significant (at 0.05) weak negative correlation between parameters of blood IFN-γ levels and right-hip bone mineral density.
oral and serumcytokines, generalized moderate chronic periodontitis, bone mineral density, osteoporosis, periodontal status in women with osteopenia, oral fluid
1. Maylyan E.A., Reznichenko N.A., Ignatenko G.A. Syvorotochnye urovni citokinov pri postmenopauzal'nom osteoporoze. Krymskiy zhurnal eksperimental'noy i klinicheskoy mediciny. 2018;8(1):36-42. [E.A. Maylyan, N.A. Reznichenko, G.A. Ignatenko. Serum levels of cytokines in postmenopausal osteoporosis. Crimean Journal of Experimental and Clinical Medicine. 2018;8(1):36-42. (In Russ.)]. https://www.elibrary.ru/item.asp?id=35310840
2. Ostrovskaya L.Yu., Zaharova N.B., Budyleva A.P., Bulkina N.V. Znachenie lokal'nogo deystviya vitamina D na immunnuyu sistemu parodonta (obzor). Stomatologiya. 2019;1:41-47. [L.Yu. Ostrovskaya, N.B. Zakharova, A.P. Budyleva, N.V. Bulkina. Significance of local effects of vitamin D on periodontium immune system (review). Dentistry. 2019;1:41-47. (In Russ.)]. doi:https://doi.org/10.17116/stomat202110006129
3. Pestrikova T.Yu., Yachinskaya T.V. Sovremennyy vzglyad na etiologiyu, patogenez, diagnostiku, profilaktiku i lechenie deficita vitamina D u zhenschin v postmenopauze (obzor literatury). Ginekologiya. 2016;1:19-23. [T.Yu. Pestrikova, T.V. Yachinskaya. Modern view on etiology, pathogenesis, prophylaxis and treatment of vitamin D deficiency for postmenopausal women (literature review). Gynecology. 2016;1:19-23. (In Russ.)]. https://gynecology.orscience.ru/2079-5831/article/view/28502
4. Solyanova N.A., Senchukova O.V., Kurilo I.N. Posledstviya deficita vitamina D u lyudey starshih vozrastov. Sovremennye problemy nauki i obrazovaniya. 2015;4:12-17. [N.A. Solyanova, O.V. Senchugova, I.N. Kurilo. Consequences of deficiency of vitamin D at people of advanced ages. Modern Problems of Science and Education. 2015;4:12-17. (In Russ.)]. https://science-education.ru/ru/article/view?id=20546
5. Sablina S.N., Elovikova T.M., Grigor'ev S.S. Rol' vitamina D v podderzhanii zdorov'ya polosti rta (literaturnyy obzor). Problemy stomatologii. 2020;16(4):25-34. [S.N. Sablina, T.M. Elovikova, S.S. Grigoriev. The role of vitamin D in maintaining oral health (literature review). Actual Problems in Dentistry. 2020;16(4):25-34. (In Russ.)]. https://doi.org/10.18481/2077-7566-20-16-4-25-34
6. Sablina S.N., Elovikova T.M., Grigor'ev S.S., Koscheev A.S. Klinicheskie parametry sostoyaniya polosti rta u zhenschin postmenopauzal'nogo perioda pri kursovom primenenii nesteroidnogo protivovospalitel'nogo preparata. Stomatologiya. 2021;100(6):29-34. [S.N. Sablina, T.M. Elovikova, S.S. Grigorjev, A.S. Koshcheev. Clinical parameters of the oral cavity in postmenopausal women during the course of a nonsteroidal anti-inflammatory drug. Dentistry. 2021;100(6):29-34. (In Russ.)]. https://doi.org/10.17116/stomat202110006129
7. Sablina S.N., Elovikova T.M., Grigor'ev S.S., Dorohina K.R., Epishova A.A., Chagay A.A. Primenenie nesteroidnyh protivovospalitel'nyh preparatov pri parodontal'noy terapii pacientov s komorbidnoy patologiey. Problemy stomatologii. 2021;17(4):13-19. [S.N. Sablina, T.M. Elovikova, S.S. Grigorjev, A.S. Koshcheev. Use of non-steroidal anti-inflammatory drugs on periodontal therapy in patients with comorbid pathology. Actual Problems in Dentistry. 2021;17(4):13-19. (In Russ.)]. doi:https://doi.org/10.18481/2077 7566 21 17 4-13-19
8. Ushakov R.V., Gerasimova T.P. Mehanizmy tkanevoy destrukcii pri parodontite. Stomatologiya. 2017;96(4):63-66. [R.V. Ushakov, T.P. Gerasimova. Mechanisms of tissue destruction in periodontal disease. Dentistry. 2017;96(4):63-66. (In Russ.)]. https://doi.org/10.17116/stomat201796463-66
9. Cepov L.M., Cepov E.L., Cepov A.L. Sochetannaya patologiya: vospalitel'nye zabolevaniya parodonta, osteoporoz, deficit vitamina D (obzor literatury). Parodontologiya. 2016;4:5-9. [L.M. Tsepov, E.L. Tsepov, A.L. Tsepov. Comorbidity: inflammatory periodontal diseases, osteoporosis, vitamin D deficiency (a review of the literature). Periodontology. 2016;4:5-9. (In Russ.)]. doi:https://doi.org/10.17116/stomat202110006129
10. Asif S., Ahmad B., Hamza S.A., Taib H., Kassim N.K., ZainuddinS.. Investigation of Salivary RANKL and OPG Levels in Periodontitis Patients at Hospital UniversitiSains Malaysia // Eur J Dent. - 2021;27. doi:https://doi.org/10.1055/s-0041-1731930.
11. Arioka M., Zhang X., Li Z., Tulu U.S. Osteoporotic changes in the periodontium impair alveolar bone healing // J Dental Res. - 2019;98(4):450-458. doi:https://doi.org/10.1177/0022034518818456.
12. Ayed M.S., Alsharif A.F., Divakar D.D., Jhugroo C., Alosaimi B., Mustafa M. Evaluating the possible association between systemic osteoporosis and periodontal disease progression in postmenopausal women // Dis Mon. - 2019;65(6):193-215. https://doi.org/10.1016/j.disamonth.2018.11.001
13. Buset S.L., Walter C., Friedmann A., Weiger R., Borgnakke W.S., Zitzmann N.U. Are periodontal diseases really silent? A systematic review of their effect on quality of life // J. Clin. Periodontol. - 2016;43:333-344. doi:https://doi.org/10.1111/jcpe.12517.
14. Hajishengallis G., Diaz P.I. Porphyromonasgingivalis: Immune subversion activities and role in periodontal dysbiosis // Current oral health reports. - 2020;7(1):12-21. https://doi.org/10.1007/s40496-020-00249-3
15. Cheng W.C., van Asten S.D., Burns L.A., Evans H.G., Walter G.J., Hashim A., Hughes F.J., Taams LS. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses // Eur J Immunol. - 2016;46(9):2211-2221. doi:https://doi.org/10.1002/eji.201545871.
16. Chapple I.L.C., Bouchard P., Cagetti M.G., Campus G., Carra M.C., Cocco F., Nibali L., Hujoel P., Laine M.L., Lingstrom P. et al. Interaction of lifestyle, behaviour or systemic diseases with dental caries and periodontal diseases: Consensus report of group 2 of the joint EFP/ORCA workshop on the boundaries between caries and periodontal diseases // J. Clin. Periodontol. - 2017;44:39-51. doi:https://doi.org/10.1111/jcpe.12685
17. Flyvbjerg A., Nielsen C.H., Hansen P.R. Comorbidity of periodontal disease: two sides of the same coin? An introduction for the clinician // J Oral Microbiol. - 2017;14;9(1):1332710. doi:https://doi.org/10.1080/20002297.2017.1332710.
18. Gomes-Filho I.S., Cruz S.S.D., Trindade S.C., Passos-Soares J.D.S., Carvalho-Filho P.C., Figueiredo A.C.M.G., Lyrio A.O., Hintz A.M., Pereira M.G., Scannapieco F. Periodontitis and respiratory diseases: A systematic review with meta-analysis // Oral Diseases. - 2020;26(2):439-446. doi:https://doi.org/10.1111/odi.13228.27
19. Qasim S., Al-Otaibi D., Al-Jasser R., Gul S.S., Zafar M.S. An Evidence-Based Update on the Molecular Mechanisms Underlying Periodontal Diseases // Int J Mol Sci. - 2020;28;21(11):3829. doi:https://doi.org/10.3390/ijms21113829.
20. Hernandez-Vigueras S., Martinez-Garriga B., Sanchez M.C., Sanz M., Estrugo-Devesa A., Vinuesa T, Lopez-Lopez J, Vinas M. Oral microbiota, periodontal status, and osteoporosis in postmenopausal females // J Periodontol. - 2016;87(2):124-133. doi:https://doi.org/10.1902/jop.2015.150365
21. Jonasson G., Rythén M. Alveolar bone loss in osteoporosis: a loaded and cellular affair? // Clin Cosmet Investig Dent. - 2016;13;8:95-103. doi:https://doi.org/10.2147/CCIDE.S92774.
22. Kim Y., Han B.G., KoGES Group Cohort profile: the Korean Genome and Epidemiology Study (KoGES) consortium // Int J Epidemiol. - 2017;46(4):1350. doi:https://doi.org/10.1093/ije/dyx105.
23. Leslie W.D., Shevroja E., Johansson H., McCloskey E.V., Harvey N.C.., Kanis J.A., Hans D. Risk-equivalent T-score adjustment for using lumbar spine trabecular bone score (TBS): the Manitoba BMD registry // Osteoporos Int. - 2018;29(3):751-758. doi:https://doi.org/10.1007/s00198-018-4405-0.
24. Manolagas S.C.. The quest for osteoporosis mechanisms and rational therapies: how far we've come, how much further we need to go // J Bone Miner Res. - 2018;33(3):371-385. doi:https://doi.org/10.1002/jbmr.3400
25. Penoni D.C., Torres S.R., Farias M.L., Fernandes T.M., Luiz R.R., Leao A.T. Association of osteoporosis and bone medication with the periodontal condition in elderly women // Osteoporos Int. - 2016;27(5):1887-1896. doi:https://doi.org/10.1007/s00198-015-3437-y
26. Richa R.Y., Puranik M.P., Shrivastava A. Association between osteoporosis and periodontal disease among postmenopausal Indian women // J Investig Clin Dent. - 2017;8(3). doi:https://doi.org/10.1111/jicd.12223.
27. Shuai Xu, Gang Zhang, Jun-feng Guo, Ying-hui Tan, Associations between osteoporosis and risk of periodontitis: A pooled analysis of observational studies // Oral Diseases. - 2020; 27(2):357-369. doi:https://doi.org/10.1111/odi.13531
28. Xiao W., Li S., Pacios S., Wang Y., Graves D.T. Bone Remodeling Under Pathological Conditions // Front Oral Biol. - 2016;18:17-27. doi:https://doi.org/10.1159/000351896
29. Watt R.G., Daly B., Allison P., Macpherson L.M.D., Venturelli R., Listl S., Weyant R.J., Mathur M.R., Guarnizo-Herreño C.C., Celeste R.K. et al. Ending the neglect of global oral health: Time for radical action // Lancet. - 2019;394:261-272. doi:https://doi.org/10.1016/S0140-6736(19)31133-X
30. Wang C.J., McCauley L.K. Osteoporosis and Periodontitis // Current osteoporosis reports. - 2016;14(6):284-291. https://doi.org/10.1007/s11914-016-0330-3
