Dushanbe, Tajikistan
Dushanbe, Tajikistan
Dushanbe, Tajikistan
Kazan', Kazan, Russian Federation
Subject. In recent years, scientific research has been carried out to create new means for stopping bleeding, developed on the basis of hybrid organic-inorganic materials that contrib-ute to ensuring effective stopping of bleeding, regardless of blood clotting factors. The purpose of the study. To conduct a morphological study in experimental conditions on animals for a comparative assessment of the hemostatic effect of dressings coated with aluminum dioxide and iron nanoparticles. Methodology. To assess the hemostatic effect, an experimental study was conducted in the morphological laboratory of the Central Research Institute of State Medical University named after Abuali Ibni Sino on male white rats of the Vistar breed, weighing from 202 to 395, divided into 3 groups: 1st (control) – a bleeding wound was observed before its sponta-neous stop; 2nd group – nanostructured Aloobene was used; 3rd – a bleeding wound was treat-ed nanostructured Fe2O3. When setting up the experiment, the following main indicators of bleeding were determined: the time of stopping bleeding and the volume of blood loss. Results. When using nanostructured Aloobene, elements of necrosis of the epidermis and dermis were noted – scab; fragments of destroyed hair follicles, hair, sebaceous and sweat glands in the scab; stagnant full-blooded vessels; inflammatory cellular infiltrates. In the der-mis and hypoderma, fullness and stagnation in the vessels, pronounced edema and destruction of fibrous-fibrous tissue were determined. Diffuse leukocyte infiltrates, foci of hemorrhage and necrosis were detected in places in the tissues. The inflammatory infiltrate forms clear contours of the de-marking line. Conclusions. The obtained results of a morphological study in experimental animals in-dicate a greater effectiveness of the blood-reducing effect when using aluminum oxide com-pared with iron oxide, the absence of toxic and teratogenic effects.
morphological study, experimental abdominal, alveolar bleeding, aluminum oxide, iron oxide
1. Susree M., Anand M. Effect of platelet concentration and calcium on plasma clot absorb-ance // International Journal of Research in Engineering and Technology. - 2016;05(06):102-104. https://ijret.org/volumes/2016v05/i06/IJRET20160506020.pdf
2. Kuznecova N.R., Vodovozova E.L. Differencial'noe svyazyvanie belkov plazmy krovi liposomami, nesuschimi v bisloe lipofil'nye prolekarstva metotreksata i melfalana. Biohimiya. 2014;79(8):999-1008. [N.R. Kuznecova, E.L. Vodovozova. Differential binding of plasma proteins by liposomes carrying lipophilic prodrugs of methotrexate and melphalan in the bilayer. Biochemistry. 2014;79(8):999-1008. (In Russ.)]. https://elibrary.ru/item.asp?id=21987660
3. Chitrakar R. et al. Phosphate adsorption on synthetic goethite and akageneite // Journal of Colloid and Interface Science. - 2006;298(2):602-608. doi:https://doi.org/10.1016/j.jcis.2005.12.054.
4. Lozhkomoev A.S. et al. Acid-base and adsorption properties of the AlOOH 2D nanostruc-tures as factors for regulating parameters of model biological solutions // Nanotechnologies in Russia. - 2016;11(7-8):506-511. https://www.infona.pl/resource/bwmeta1.element.springer-doi-10_1134-S1995078016040108
5. Chen J. et al. Preparation of a partially carboxymethylated cotton gauze and study of its hemostatic properties // Journal of Mechanical Behavior of Biomedical Materials. - 2016;(62):407-416. doi:https://doi.org/10.1016/j.jmbbm.2016.04.018.
6. Hexig B. et al. Safety evaluation of surgical materials by cytotoxicity testing // Journal of Artificial Organs. - 2008;11(4):204-211. doi:https://doi.org/10.1007/s10047-008-0429-0.
7. Lozhkomoev A.S. et al. Application of Hierarchical Nanostructured Aluminum Oxyhydroxide for Bleeding Control. AIP Conference Proceedings 2019. https://doi.org/10.1063/1.5132066
8. Ryabyh L.A., Aver'yanov S.V., Kazakova E.A. Profilaktika lunochkovogo krovoteche-niya u bol'nyh pri soputstvuyuschih somaticheskih zabolevaniyah. Sbornik statey nauch-no-prakticheskoy konferencii Privolzhskogo (Kazanskogo) federal'nogo universiteta «Kachestvo okazaniya medicinskoy stomatologicheskoy pomoschi: sposoby dostizheniya, kriterii i metody ocenki». Kazan', 17-18 marta. 2016:142-146. [L.A. Ryabykh, S.V. Averyanov, E.A. Kazakova. Prevention of alveolar bleeding in patients with concomitant somatic diseases. Collection of articles of the scientific-practical conference of the Volga (Kazan) Federal University "The quality of medical dental care: ways to achieve, criteria and methods of evaluation." Kazan, March 17-18. 2016:142-146. (In Russ.)]. https://elibrary.ru/item.asp?id=25788741
9. Kardanova K.H. i dr. Profilaktika i lechenie oslozhneniy posle operacii udaleniya zuba u pacientov s razlichnym urovnem gigieny polosti rta. Uspehi sovremennoy nauki. 2017;9(4):194-198. [K.H. Kardanova et al. Prevention and treatment of complications after tooth extraction surgery in patients with different levels of oral hygiene. Successes of modern science. 2017;9(4):194-198. (In Russ.)]. https://elibrary.ru/item.asp?id=29318647
10. Velichko E.V., Gun'ko V.I. Planirovanie i posledovatel'nost' deystviy pri pro-vedenii hirurgicheskih stomatologicheskih manipulyaciy bol'nym, prinimayuschim varfarin. Nauchno-prakticheskiy zhurnal Vrach-aspirant. 2010;(6):451-452. [E.V. Velichko, V.I. Gun'ko. Planning and sequence of actions during surgical dental manipulations for patients taking warfarin. Scientific and practical journal Postgraduate doctor. 2010;(6):451-452. (In Russ.)]. https://elibrary.ru/item.asp?id=15589529
11. Permyakova N.E. Kompleksnaya profilaktika vtorichnyh lunochkovyh krovotecheniy posle udalenie zubov u bol'nyh s gemofiliey : diss. ... kand. med. nauk. Perm', 2005. [N.E. Permyakova. Comprehensive prevention of secondary alveolar bleeding after tooth extraction in patients with hemophilia: diss. ... cand. med. sciences. Perm, 2005. (In Russ.)]. https://elibrary.ru/item.asp?id=16176896
