Tver, Tver, Russian Federation
Tver, Tver, Russian Federation
Tver, Tver, Russian Federation
Tver, Tver, Russian Federation
Tver, Tver, Russian Federation
Researchers are currently paying more and more attention to the combined pathology of the tooth pulp and periodontal. At the same time, the peculiarities of the structure of the alveolar bone, the cement of the tooth root and the root canal system that cause their interrelation and mutual influence remain insufficiently studied. The main elements of the connection between the pulp and periodontal tissues are not only apical openings and additional channels, but also other ways of interaction, the study of which is of great interest for understanding the features of pathogenesis, prognosis and treatment of combined diseases of the tooth pulp and periodontal. The aim of the study is to identify the topographical and anatomical features of the structure of the roots of teeth of different functional groups and the surfaces of their holes when studying on human cadaver material. The hypothesis of the study is that the number of openings of the Haversov channels and their localization in the moons are due to the functional affiliation of the teeth and they have their own characteristics. Material and methods. The study was conducted on jawbones with teeth obtained from embalmed corpses of adults of different genders and ages. After appropriate preparation, at the first stage, microscopy of the alveolar surfaces was performed, followed by morphometric analysis of the photos. At the second stage, scanning electron microscopy (SEM) of the surfaces of the roots of teeth previously extracted from the same wells of the jaws was performed. Results and discussion. All the preparations were systematized according to the number and topography of the identified holes of the Haversov channels. Because of the assessment of morphometric indicators, the number of holes, the density of their location and the diameter were determined, depending on the functional affiliation of the teeth. The analysis of microphotographs obtained because of SEM allowed us to determine the structural features of the surfaces of the roots of the teeth. Conclusions. The number, density and diameter of the holes of the Haversov channels are determined by the functional affiliation of the tooth. With the help of SEM, micro cracks and areas of root dentin exposure were found on the surfaces of the roots of the teeth for the first time, which can contribute to the translocation of pathogenic microbiota from the periodontal pocket to the pulp of the tooth and vice versa.
pulp, periodontal disease, structure, relationship, pulp-periodontal lesions, pulpitis, chronic generalized periodontitis
1. Moroz P.V. Endo-parodontal'nyy sindrom: anatomicheskie predposylki razvitiya, etiologiya, klassifikaciya i taktika lecheniya. Institut stomatologii. 2014;2(63):91-94. [P.V. Moroz. Endo-periodontal syndrome: anatomical prerequisite for development, etiology, classification and treatment. Institute of Dentistry. 2014;2(63):91-94. (In Russ.)]. https://www.elibrary.ru/item.asp?id=22133961
2. Singh P. Endo-perio dilemma: a brief review // Dental research journal. - 2011;1(8):39-47. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177380/
3. Moroz P.V., Iordanishvili A.K. Faktory riska vozniknoveniya i prichiny nizkoy effektivnosti lecheniya endodonto-parodontal'nyh porazheniy. Endodontiya today. 2018;1:35-41. [P.V. Moroz, A.K. Iordanishvili. Risk factors and causes of low efficiency of treatment of lesions endo-periodontal lesions. Endodontics today. 2018;1:35-41. (In Russ.)]. https://www.elibrary.ru/item.asp?id=35001913
4. Gong A.-X., Zhang J.-H., Li J., Wu J., Wang L., Miao D.-S. Comparison of gene expression profiles between dental pulp and periodontal ligament tissues in humans // International journal of molecular medicine. - 2017;3(40):647-660. https://doi.org/10.3892/ijmm.2017.3065
5. Vol'f G.F., Rateychak E.M., Rateychak K. Parodontologiya. Moskva : MEDpress-inform. 2014:548. [G.F. Vol'f, Je.M. Ratejchak, K. Ratejchak. Periodontics. Moscow : MEDpress-inform. 2014:548. (In Russ.)].
6. Kopytov A.A., Lyubushkin R.A., Kolesnikov D.A., Tverskoy A.V., Tyschenko N.S. Morfometricheskie harakteristiki ust'ev por kortikal'noy plastinki v oblasti molyarov nizhney chelyusti. Parodontologiya. 2014;1(70);(19):13-19. [A.A. Kopytov, R.A. Ljubushkin, D.A. Kolesnikov, A.V. Tverskoj, N.S. Tyshhenko. Morphometric characteristics pore mouths of the cortical bone in the mandibular molars. Periodontics. 2014;1(70);(19):13-19. (In Russ.)]. https://www.elibrary.ru/item.asp?id=21479347
7. Rumyancev V.A., Polunina O.S., Opeshko V.V., Moiseev D.A. Nanoimpregnaciya dentina zubov pri eksperimental'nom lechenii kariesa: ocenka s pomosch'yu elektronnoy mikroskopii. Parodontologiya. 2016;3(80);(21):68-71. [V.A. Rumjancev, O.S. Polunina, V.V. Opeshko, D.A. Moiseev. Evaluation nanoimpregnation dentin of the teeth with an electron microscope in experimental treatment of caries. Periodontics. 2016;3(80);(21):68-71. (In Russ.)].
8. Rumyancev V.A., Fedotova T.A., Zablockaya M.V., Yusupova Yu.I., Ryabikov M.D., Moiseev D.A. Novyy metod kompleksnogo lecheniya endodonto-parodontal'nyh porazheniy s pomosch'yu nanoimpregnacii i kupral-kyuretazha. Verhnevolzhskiy medicinskiy zhurnal. 2017;4(16):4-9. [V.A. Rumyantsev, T.A. Fedotova, M.V. Zablotskaya, Yu.I. Yusupova, M.D. Ryabikov, D.A. Moiseev. New method of complex treatment of endodont-parodontal diseases by nanoimpregnation and cupral-curettage. Upper Volga Medical Journal. 2017;4(16):4-9. (In Russ.)]. https://www.elibrary.ru/item.asp?id=32239360.
9. Denisova Yu.L., Gerasimovich A.I., Rosenik N.I. Kliniko-patomorfologicheskie izmeneniya tkaney periodonta u pacientov s endoperiodontitom. Medicinskiy zhurnal. 2018;2(64):42-47. [Yu.L. Denisova, A.I. Gerasimovich, N.I. Rosenik. Specific clinical and pathomorphological features of periodontal tissues in patients with endoperiodontitis. Medical journal. 2018;2(64):42-47. (In Russ.)]. https://www.elibrary.ru/item.asp?id=35126388
10. Makeeva I.M., Turkina A.Yu. Kompleksnoe lechenie endodonto-parodontal'nyh porazheniy: klinicheskie primery. Dental Tribune Russia. 2016;3-4:7. [I.M. Makeeva, A.Yu. Turkina. Complex treatment of endodonto-periodontal lesions: clinical examples. Dental Tribune Russia. 2016;3-4:7. (In Russ.)]. https://www.dental-tribune.com/epaper/dt-russia/dt-russia-no-34-2016-0416-%5B07-07%5D.pdf
11. Chisini L.A., Conde M.M., Grazioli G., Martin A.S., Carvalho R.V., Sartori L.M., Demarco F.F. Bone, periodontal and dental pulp regeneration in dentistry: a systematic scoping review // Brazilian dental journal. - 2019;2(30):77-95. https://doi.org/10.1590/0103-6440201902053
12. Galeeva Z.R., Muhamedzhanova L.R., Gruber N.M. Tubulyarnyy put' mikrobnoy invazii u pacientov s endo-parodontal'nymi ochagami infekcii. Prakticheskaya medicina. 2012;8(64);2:31-33. [Z.R. Galeeva, L.R. Muhamedzhanova, N.M. Gruber. The tubular pathway of microbial invasion in patients with endo-periodontal foci of infection. Practical medicine. 2012;8(64);2:31-33. (In Russ.)]. https://cyberleninka.ru/article/n/tubulyarny-put-mikrobnoy-invazii-u-patsientov-s-endoparodontalnymi-ochagami-infektsii
13. Foster B.L. Methods for studying tooth root cementum by light microscopy // International journal of oral science. - 2012;(4):119-128. https://doi.org/10.1038/ijos.2012.57
14. Aboulfadl H., Hulliger J. Absolute polarity determination of teeth cementum by phase sensitive second harmonic generation microscopy // Journal of structural biology. - 2015;1(192):67-75. https://doi.org/10.1016/j.jsb.2015.08.011
15. Cho H.J., Jeon J.Y., Ahn S.J., Lee S.W., Chung J.R., Park C.J., Hwang K.G. The preliminary study for three-dimensional alveolar bone morphologic characteristics for alveolar bone restoration // Maxillofacial plastic and reconstructive surgery. - 2019;1(41):33. https://doi.org/10.1186/s40902-019-0216-2
16. Alquthami H., Almalik A.M., Alzahrani F.F., Badawi L. Successful management of teeth with different types of endodontic-periodontal lesions // Case reports in dentistry. - 2018;2018:7084245. https://doi.org/10.1155/2018/7084245
17. Gautam S., Galgali S.R., Sheethal H.S., Priya N.S. Pulpal changes associated with advanced periodontal disease: a histopathological study // Journal of oral and maxillofacial pathology: JOMFP. - 2017;1(21):58-63. https://doi.org/10.4103/0973-029X.203795
18. Rumyancev V.A., Nekrasov A.V., Moiseev D.A., Zadorozhnyy D.V., Pankin P.I. Bioplenka v endodontii. Chast' II. Metody bor'by s bioplenkoy pri endodonticheskom lechenii zubov (obzor literatury). Endodontiya today. 2018;2:38-42. [V.A. Rumyantsev, A.V. Nekrasov, D.A. Moiseev, D.V. Zadorozhny, P.I. Pankin. Biofilm in endodontics. Part II. Methods of combating biofilm in endodontic dental treatment (literature review). Endodontics today. 2018;2:38-42. (In Russ.)]. https://doi.org/10.25636/PMP.2.2018.2.8
19. Couve E., Schmachtenberg O. Schwann cell responses and plasticity in different dental pulp scenarios // Frontiers in cellular neuroscience. - 2018;12;299:1-8. https://doi.org/10.3389/fncel.2018.00299
20. Faustov L.A., Leont'ev V.K., Popkov V.L., Grechishnikov V.V., Sycheva N.L. Ul'trastrukturnaya harakteristika tverdyh tkaney kornya zuba pri pul'pitah. Fenomen formirovaniya v dentine inficirovannyh ochagov destrukcii. Nauchnye vedomosti Belgorodskogo gosuniversiteta. 2011;6(111):93-99. [L.A. Faustov, V.K. Leontiev, V.L. Popkov, V.V. Grechishnikov, N.L. Sycheva. Ultrastructural characteristics of hard tkaney kornya tooth root tissues in pulpitis. The phenomenon of formation of infected foci of destruction in dentin. Scientific bulletin of Belgorod State University. 2011;6(111):93-99. (In Russ.)]. https://cyberleninka.ru/article/n/ultrastrukturnaya-harakteristika-tverdyh-tkaney-kornya-zuba-pri-pulpitah-fenomen-formirovaniya-v-dentine-infitsirovannyh-ochagov
21. Usmanova I.N., Gerasimova L.P., Kabirova M.F. i dr. Vzaimosvyaz' klinicheskih i morfologicheskih izmeneniy s faktorami riska razvitiya vospalitel'nyh zabolevaniy parodonta u lic molodogo vozrasta. Klinicheskaya stomatologiya. 2017;4(84):34-39. [I.N. Usmanova, L.P. Gerasimova, M.F. Kabirova et al. The relationship of clinical and morphological signs with risk factors for the development of inflammatory periodontal diseases at young age. Clinical dentistry. 2017;4(84):34-39. (In Russ.)]. https://www.elibrary.ru/item.asp?id=30684147
22. Karakov K.G., Hachaturyan E.E., Uzdenov M.B., Uzdenova L.H., Vanchenko N.B., Hachaturyan A.E., Curova M.A. Sposob lecheniya hronicheskogo generalizovannogo parodontita legkoy i sredney stepeney tyazhesti. Problemy stomatologii. 2020;2(16):53-58. [K.G. Karakov, E.E. Khachaturian, M.B. Uzdenov, L.H. Uzdenova, N.B. Ivanchenko, A.E. Khachaturian, M.A. Surkova. Method of treatment of chronic generalized periodontitis of mild and moderate severity. Actual problems in dentistry. 2020;2(16):53-58. (In Russ.)]. https://doi.org/10.18481/2077-7566-20-16-2-53-58
23. Usmanova I.N., Gerasimova L.P., Kabirova M.F. i dr. Diagnosticheskie kriterii hronicheskogo gingivita i parodontita u lic molodogo vozrasta. Parodontologiya. 2014;19;4(73):44-49. [I.N. Usmanova, L.P. Gerasimova, M.F. Kabirova et al. Early diagnosis of chronic gingivitis and periodontal disease in persons of young age. Periodontics. 2014;19;4(73):44-49. (In Russ.)]. https://www.elibrary.ru/item.asp?id=22872761
24. Bekirova L.G., Dzhafarova A.R., Yusubova Sh.R., Alieva G.G., Ibragimova L.K., Damirchieva M.V. Povyshenie effektivnosti kompleksnogo lecheniya vospalitel'nyh zabolevaniy parodonta. Problemy stomatologii. 2020;4(15):84-91. [L.G. Bekirova, A.R. Jafarova, Sh.R. Yusupova, G.G. Alieva, L.K. Ibragimova, M.V. Damirchieva. Improving the effectiveness of complex treatment of inflammatory periodontal diseases. Actual problems in dentistry. 2020;4(15):84-91. (In Russ.)]. https://doi.org/10.18481/2077-7566-2019-15-4-84-91
25. Usmanova I.N., Gerasimova L.P., Kabirova M.F. i dr. Kliniko-morfologicheskie izmeneniya tkaney parodonta, obuslovlennye nalichiem drozhzhepodobnyh gribov roda Candida u lic molodogo vozrasta. Parodontologiya. 2015;20;3(76):62-66. [I.N. Usmanova, L.P. Gerasimova, M.F. Kabirova et al. Clinical and morphological changes in tissues periodontitis in young people due to the presence of yeast-like fungi of the genus Candida. Periodontics. 2015;20;3(76):62-66. (In Russ.)]. https://www.elibrary.ru/item.asp?id=24365637
