from 01.01.2004 to 01.01.2018
Perm', Perm, Russian Federation
Perm', Perm, Russian Federation
Perm, Perm, Russian Federation
from 01.01.2015 until now
FGBOU VO PGMU im. akademika E.A. Vagnera Minzdrava Rossii
Perm', Russian Federation
Russian Federation
Perm', Perm, Russian Federation
UDK 61 Медицина. Охрана здоровья
GRNTI 76.03 Медико-биологические дисциплины
GRNTI 76.29 Клиническая медицина
OKSO 31.02.06 Стоматология профилактическая
BBK 566 Стоматология
BISAC HEA040000 Oral Health
Subject. Changes in the activity of certain saliva enzymes, due to certain factors and in certain clinical conditions of the human body, can affect oral hygiene, as well as indirectly on the activity of the carious process. Of particular interest is the enzyme — alpha-amylase, which is due to its ability to cleave dextrans and levans, which are the basis of the matrix of the bacterial film. The article presents data on changes in the activity of saliva alpha-amylase in microecological disorders in the intestine and acute respiratory viral diseases, as well as its effect on the level of oral hygiene. The goal — the assessment of changes in the activity of saliva alpha-amylase in microecological disorders in the intestine and acute respiratory diseases, taking into account the level of oral hygiene. Methods. To determine the activity of alpha-amylase, a set of reagents "Amylase-Vital" was used according to the manufacturer's instructions, proportionally reducing the volume of reagents for the procedure in titration plates. The volunteers, divided into groups with severe acute respiratory syndrome, microecological intestinal disorders, and a control group, received mixed saliva and evaluated their oral hygiene status. Results. The inverse relationship between the severity of intoxication syndrome in severe acute respiratory syndrome and the level of saliva alpha-amylase activity is shown. In addition, a correlation was established between an increase in human body temperature and a decrease in the activity of alpha-amylase. There is also a link between the presence of decompensated intestinal dysbiosis in humans and the activity of the enzyme. The dependence between the decrease in the activity of the studied enzyme and the increase in biofilm formation on the organs of the oral cavity was revealed. Conclusions and Relevance. The conducted studies open up a promising direction for the development of additional diagnostic criteria based on the determination of the activity of saliva alpha-amylase. In addition, the data obtained on the deterioration of oral hygiene indicate the need for a comprehensive approach to the treatment of patients in this category with the mandatory involvement of a dentist.
saliva, alpha-amylase, biofilm, intestinal dysbiosis, severe acute respiratory syndrome, oral hygiene
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