EFFECT OF THE INTRODUCTION OF NANODIMENSIONAL TITANIUM DIOXIDE ON THE FORMATION OF STAPHYLOCOCCUS EPIDERMIDIS 33 FILMS GROWN ON VERTEX THERMOSENS SUBSTRATES
Rubrics: STOMATOLOGICAL MATERIAL SCIENCES
Authors:
1.
Perm State Medical University named after Academician E. A. Vagner
(Orthopedic Dentistry Department, Professor of the Department)
employee
Perm, Perm, Russian Federation
employee
Perm, Perm, Russian Federation
2.
Perm State Medical University named after Academician E. A. Vagner
(department of orthopedic stomatology, Professor of the Department)
employee from 01.01.2004 to 01.01.2018
Perm', Perm, Russian Federation
employee from 01.01.2004 to 01.01.2018
Perm', Perm, Russian Federation
3.
Institute of ecology and genetics of microorganisms
Perm', Perm, Russian Federation
Perm', Perm, Russian Federation
4.
Institute of ecology and genetics of microorganisms of UB RAS
Perm', Perm, Russian Federation
Perm', Perm, Russian Federation
Type:
Article
Pages:
from 41
to 45
Status:
Published
Received:
25.03.2017
Accepted:
25.03.2017
Published:
25.03.2017
Subject area:
GRNTI 76.29 Клиническая медицина
Language:
Russian
Keywords:
Staphylococcus epidermidis 33, dioksid titana, poliamid, mikrobnaya plenka, ingibirovanie
Abstract (English):
Predmet. Problema obrazovaniya mikrobnyh plenok na konstrukcionnyh materialah ne teryaet svoey aktual'nosti nesmotrya na sovremennyy uroven' razvitiya stomatologicheskogo materialovedeniya. Pri etom naibol'shiy risk predstavlyayut pacienty s priobretennymi defektami i deformaciyami chelyustno-licevoy oblasti. V bol'shinstve sluchaev eto svyazano s zatrudneniem provedeniya gigienicheskih meropriyatiy i slozhnoy topografiey defekta i, sootvetstvenno, slozhno-chelyustnogo proteza. V stat'e rassmatrivaetsya variant ingibirovaniya obrazovaniya mikrobnoy plenki na bazisnom konstrukcionnom materiale putem modifikacii ego sostava. Cel'. Eksperimental'no-laboratornoe izuchenie vozmozhnosti ingibirovaniya obrazovaniya bioplenki Staphylococcus epidermidis 33 na poliamidnom konstrukcionnom materiale Vertex ThermoSens pri vvedenii v ego sostav nanorazmernogo dioksida titana. Metodologiya. Issledovanie rosta mikrobnyh bioplenok provodili na obrazcah poliamidnogo materiala Vertex ThermoSens. Izuchenie biomassy plenok provodili putem izmereniya svyazyvaniya kletkami bakteriy i matriksom bioplenok krasitelya – gencianvioleta. Kolichestvo zhiznesposobnyh kletok v bioplenkah vyyavlyali po urovnyu vosstanovleniya vodorastvorimogo tetrazoliya. Posleduyuschuyu detekciyu opticheskoy plotnosti izmeryali na spektrofotometre. Rezul'taty. Eksperimental'nye obrazcy Vertex ThermoSens s vvedennym v sostav nanorazmernym dioksidom titana pokazali bol'shuyu rezistentnost' v otnoshenii obschey biomassy i zhivyh kletok posle 48-chasovogo kul'tivirovaniya mikrobnoy plenki Staphylococcus epidermidis 33, imeya dostovernoe statisticheski znachimoe snizhenie v dva i bolee raza. Vyvody. Vklyuchenie v sostav bazisnogo konstrukcionnogo materiala Vertex ThermoSens nanorazmernogo dioksida titana v kolichestve do 1 mas.% privodit k ugneteniyu plenkoobrazuyuschey sposobnosti Staphylococcus epidermidis 33 na poverhnosti eksperimental'nyh obrazcov kak v otnoshenii obschey biomassy, tak i zhivyh kletok. Dannyy fakt imeet prakticheskoe znachenie dlya ispol'zovaniya poluchennyh rezul'tatov eksperimenta v klinicheskoy stomatologii v kachestve sposoba ingibirovaniya obrazovaniya mikrobnoy plenki na stomatologicheskom bazisnom polimernom materiale na etape izgotovleniya s'emnyh konstrukciy slozhno-chelyustnyh protezov i apparatov.
Predmet. Problema obrazovaniya mikrobnyh plenok na konstrukcionnyh materialah ne teryaet svoey aktual'nosti nesmotrya na sovremennyy uroven' razvitiya stomatologicheskogo materialovedeniya. Pri etom naibol'shiy risk predstavlyayut pacienty s priobretennymi defektami i deformaciyami chelyustno-licevoy oblasti. V bol'shinstve sluchaev eto svyazano s zatrudneniem provedeniya gigienicheskih meropriyatiy i slozhnoy topografiey defekta i, sootvetstvenno, slozhno-chelyustnogo proteza. V stat'e rassmatrivaetsya variant ingibirovaniya obrazovaniya mikrobnoy plenki na bazisnom konstrukcionnom materiale putem modifikacii ego sostava. Cel'. Eksperimental'no-laboratornoe izuchenie vozmozhnosti ingibirovaniya obrazovaniya bioplenki Staphylococcus epidermidis 33 na poliamidnom konstrukcionnom materiale Vertex ThermoSens pri vvedenii v ego sostav nanorazmernogo dioksida titana. Metodologiya. Issledovanie rosta mikrobnyh bioplenok provodili na obrazcah poliamidnogo materiala Vertex ThermoSens. Izuchenie biomassy plenok provodili putem izmereniya svyazyvaniya kletkami bakteriy i matriksom bioplenok krasitelya – gencianvioleta. Kolichestvo zhiznesposobnyh kletok v bioplenkah vyyavlyali po urovnyu vosstanovleniya vodorastvorimogo tetrazoliya. Posleduyuschuyu detekciyu opticheskoy plotnosti izmeryali na spektrofotometre. Rezul'taty. Eksperimental'nye obrazcy Vertex ThermoSens s vvedennym v sostav nanorazmernym dioksidom titana pokazali bol'shuyu rezistentnost' v otnoshenii obschey biomassy i zhivyh kletok posle 48-chasovogo kul'tivirovaniya mikrobnoy plenki Staphylococcus epidermidis 33, imeya dostovernoe statisticheski znachimoe snizhenie v dva i bolee raza. Vyvody. Vklyuchenie v sostav bazisnogo konstrukcionnogo materiala Vertex ThermoSens nanorazmernogo dioksida titana v kolichestve do 1 mas.% privodit k ugneteniyu plenkoobrazuyuschey sposobnosti Staphylococcus epidermidis 33 na poverhnosti eksperimental'nyh obrazcov kak v otnoshenii obschey biomassy, tak i zhivyh kletok. Dannyy fakt imeet prakticheskoe znachenie dlya ispol'zovaniya poluchennyh rezul'tatov eksperimenta v klinicheskoy stomatologii v kachestve sposoba ingibirovaniya obrazovaniya mikrobnoy plenki na stomatologicheskom bazisnom polimernom materiale na etape izgotovleniya s'emnyh konstrukciy slozhno-chelyustnyh protezov i apparatov.
Keywords:
Staphylococcus epidermidis 33, dioksid titana, poliamid, mikrobnaya plenka, ingibirovanie
Staphylococcus epidermidis 33, dioksid titana, poliamid, mikrobnaya plenka, ingibirovanie
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