ASSESSMENT OF DENTAL AND PERIODONTAL TISSUES BASED ON CLINICAL PARAMETERS AND LABORATORY INDICATORS OF PHOSPHORUS-CALCIUM METABOLISM IN MIXED SALIVA IN CHILDREN WITH HYPOPHOSPHATASIA

Irina Alekseeva; Larisa Kisel'nikova; Oleg Admakin; Irina Danilova; Irina Gette; Ksenia Sokolova; Zhanna Belaya; Natalia Kalinchenko

doi:10.18481/2077-7566-2025-21-3-160-169

VAK Russia 3.1.7 UDC 616.31

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ASSESSMENT OF DENTAL AND PERIODONTAL TISSUES BASED ON CLINICAL PARAMETERS AND LABORATORY INDICATORS OF PHOSPHORUS-CALCIUM METABOLISM IN MIXED SALIVA IN CHILDREN WITH HYPOPHOSPHATASIA

Published in Actual problems in dentistry · Volume 21, Issue 3 · Pages 160–169 · Rubric: PEDIATRIC DENTISTRY, PROPHILAXIS AND ORTODONTICS

DOI: https://doi.org/10.18481/2077-7566-2025-21-3-160-169

Received: 24.10.2025 Accepted: 29.10.2025 Published: 29.10.2025 Language of publication: RUS

Authors

Irina Alekseeva

ORCID

Larisa Kisel'nikova

ORCID

Oleg Admakin

Irina Danilova

ORCID

et al.

Irina Gette

Ksenia Sokolova

Zhanna Belaya

Natalia Kalinchenko

Irina Alekseeva

Academic degree: doctor of medical sciences

Moscow State University of Medicine and Dentistry Assistant of the Department of Pediatric Dentistry · Department of Pediatric Dentistry · Moscow

ORCID

Larisa Kisel'nikova

Academic degree: doctor of medical sciences

Science rang: professor

Russian University of Medicine Head of the Department · Department of the Pediatric Therapeutic Dentistry · Russian Federation · Moskow

ORCID

Oleg Admakin

Academic degree: doctor of medical sciences

Science rang: professor

Russian University of Medicine Deputy Director for Academic Affairs · A.I. Evdokimov Scientific Research Institute of Dentistry · Russian Federation · Moscow

Irina Danilova

Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences Russian Federation · Yekaterinburg

ORCID

Irina Gette

Academic degree: candidate of sciences in biology

Institute of Immunology and Physiology Laboratory of Morphology and Biochemistry · Laboratory of Morphology and Biochemistry · Russian Federation · Ekaterinburg

Ksenia Sokolova

Academic degree: candidate of sciences in biology

Institute of Immunology and Physiology Senior Researcher · Laboratory of Morphology and Biochemistry · Russian Federation · Ekaterinburg

Zhanna Belaya

Academic degree: doctor of medical sciences

Science rang: professor

National Medical Research Center of Endocrinology named after Academician I.I. Dedov Head of the Department · Osteoporosis and Osteopathy Department · Russian Federation · Moscow

Natalia Kalinchenko

Academic degree: candidate of medical sciences

National Medical Research Center of Endocrinology named after. Academician I.I. Dedov Associate Professor · Department of Pediatric Endocrinology and Diabetology · Russian Federation · Moscow

ABSTRACT

Relevance. Evaluation of mineral metabolism markers in mixed saliva may be a non-invasive method for diagnosing potential disorders and developing strategies for the prevention and treatment of dental diseases in children with hypophosphatasia (HPP). Objective: To determine the content of vitamin D, osteocalcin (OCC), osteoprotegerin (OPT), bone-specific alkaline phosphatase (BSAP), and parathyroid hormone (PTH) in mixed saliva and to study their relationships with some clinical parameters of dental status in children with HPP. Materials and Methods. Twenty children with HPP, aged 6–17 years, and 20 healthy children of the same age were examined. Caries intensity and structural and functional caries resistance of enamel in permanent teeth were assessed using the KPU index and the TER test, respectively; periodontal tissue condition was determined using the PMA index. The content of vitamin D, TBPC, OPT, and IAP were determined using enzyme-linked immunosorbent assay (ELISA). Results. Clinical evaluation of key dental parameters in children with HPP and healthy children revealed insufficient oral hygiene associated with an increase in caries in permanent teeth and the presence of moderate gingivitis. In the laboratory phase of the study, a statistically significant difference (2-fold) in the mixed saliva of children with HPP was found in a two-fold decrease in IAP secretion. Reduced levels of vitamin D concentration and OPT minute secretion in mixed saliva in children with HPP are associated with the development of caries in permanent teeth and soft tissue inflammation. Conclusions. The identified changes in biochemical parameters (IBP, vitamin D, OPT, and OPT) in mixed saliva allow us to recommend them as markers for identifying the risk of impaired formation and mineralization of dental and periodontal tissues in children with hypophosphatasia.

KEYWORDS

children with hypophosphatasia, mineral metabolism markers, mixed saliva, interaction, assessment of key dental indicators

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GOST

Alekseeva I. A., Kisel'nikova L. P., Admakin O. I., Danilova I. G., Gette I. F., Sokolova K. V., Belaya Z. E., Kalinchenko N. Y. ASSESSMENT OF DENTAL AND PERIODONTAL TISSUES BASED ON CLINICAL PARAMETERS AND LABORATORY INDICATORS OF PHOSPHORUS-CALCIUM METABOLISM IN MIXED SALIVA IN CHILDREN WITH HYPOPHOSPHATASIA // Actual problems in dentistry. 2025. no. 3. pp. 160-169. DOI: https://doi.org/10.18481/2077-7566-2025-21-3-160-169. (Date of access 24.10.2026).

MLA

Irina Alekseeva, Kisel'nikova L. P., Admakin O. I., Danilova I. G., Gette I. F., Sokolova K. V., Belaya Z. E., Kalinchenko N. Y.. "ASSESSMENT OF DENTAL AND PERIODONTAL TISSUES BASED ON CLINICAL PARAMETERS AND LABORATORY INDICATORS OF PHOSPHORUS-CALCIUM METABOLISM IN MIXED SALIVA IN CHILDREN WITH HYPOPHOSPHATASIA." Actual problems in dentistry 21.3 (2025): 160-169. DOI: https://doi.org/10.18481/2077-7566-2025-21-3-160-169.

APA

Alekseeva I. A., Kisel'nikova L. P., Admakin O. I., Danilova I. G., Gette I. F., Sokolova K. V., Belaya Z. E., Kalinchenko N. Y. (2025). ASSESSMENT OF DENTAL AND PERIODONTAL TISSUES BASED ON CLINICAL PARAMETERS AND LABORATORY INDICATORS OF PHOSPHORUS-CALCIUM METABOLISM IN MIXED SALIVA IN CHILDREN WITH HYPOPHOSPHATASIA. Actual problems in dentistry, 21(3), 160-169. https://doi.org/10.18481/2077-7566-2025-21-3-160-169

Chicago

Irina Alekseeva, Kisel'nikova L. P., Admakin O. I., Danilova I. G., Gette I. F., Sokolova K. V., Belaya Z. E., Kalinchenko N. Y.. "ASSESSMENT OF DENTAL AND PERIODONTAL TISSUES BASED ON CLINICAL PARAMETERS AND LABORATORY INDICATORS OF PHOSPHORUS-CALCIUM METABOLISM IN MIXED SALIVA IN CHILDREN WITH HYPOPHOSPHATASIA." Actual problems in dentistry 21, no. 3 (2025): 160-169. https://doi.org/10.18481/2077-7566-2025-21-3-160-169.

WIKI

{{cite journal | author = Alekseeva I. A., Kisel'nikova L. P., Admakin O. I., Danilova I. G., Gette I. F., Sokolova K. V., Belaya Z. E., Kalinchenko N. Y. | title = ASSESSMENT OF DENTAL AND PERIODONTAL TISSUES BASED ON CLINICAL PARAMETERS AND LABORATORY INDICATORS OF PHOSPHORUS-CALCIUM METABOLISM IN MIXED SALIVA IN CHILDREN WITH HYPOPHOSPHATASIA | journal = Actual problems in dentistry | year = 2025 | volume = 21 | issue = 3 | pages = 160-169 | doi = 10.18481/2077-7566-2025-21-3-160-169 }}

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ASSESSMENT OF DENTAL AND PERIODONTAL TISSUES BASED ON CLINICAL PARAMETERS AND LABORATORY INDICATORS OF PHOSPHORUS-CALCIUM METABOLISM IN MIXED SALIVA IN CHILDREN WITH HYPOPHOSPHATASIA

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