EXPERIMENTAL RATIONALE FOR THE APPLICATION OF BIORESORBABLE PERSONALIZED COLLAGEN MEMBRANES FOR CLOSURE OF DEFECTS IN THE ORAL MUCOSA

Mikhail Gostev; Svetlana Tarasenko; Sabina Kazumyan; Ekaterina Diachkova; Anna Usanova; Alexey Fayzullin; Petr Timashev; Elena Sadchkova

doi:10.18481/2077-7566-2023-19-4-77-82

VAK Russia 3.1.7 UDC 616.31

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EXPERIMENTAL RATIONALE FOR THE APPLICATION OF BIORESORBABLE PERSONALIZED COLLAGEN MEMBRANES FOR CLOSURE OF DEFECTS IN THE ORAL MUCOSA

Published in Actual problems in dentistry · Volume 19, Issue 4 · Pages 77–82 · Rubric: SURGICAL DENTISTRY AND IMPLANTOLOGY

DOI: https://doi.org/10.18481/2077-7566-2023-19-4-77-82

Received: 03.12.2023 Accepted: 10.12.2023 Published: 28.12.2023 Language of publication: RUS

Authors

Mikhail Gostev

Svetlana Tarasenko

Sabina Kazumyan

Ekaterina Diachkova

et al.

Anna Usanova

Alexey Fayzullin

Petr Timashev

Elena Sadchkova

Mikhail Gostev

First Moscow State Medical University named after I.M. Sechenov Assistant of the department · Department of Surgical Dentistry · Russian Federation · Moscow

Svetlana Tarasenko

Academic degree: doctor of medical sciences

Science rang: professor

First Moscow State Medical University named after I.M. Sechenov Head of Department · Department of Oral Surgery of Borovskiy Institute of Dentistry · Russian Federation · Moscow

Sabina Kazumyan

First Moscow State Medical University named after I.M. Sechenov Department of Surgical Dentistry · Russian Federation · Moscow

Ekaterina Diachkova

Academic degree: candidate of medical sciences

First Moscow State Medical University named after I.M. Sechenov associate professor · Department of Oral Surgery of Borovskiy Institute of Dentistry · Russian Federation · Moscow

Anna Usanova

First Moscow State Medical University named after I.M. Sechenov junior researcher · Center for Innovative Collagen Development of the Institute of Regenerative Medicine · Russian Federation · Moscow

Alexey Fayzullin

Academic degree: candidate of medical sciences

First Moscow State Medical University named after I.M. Sechenov Head of the Laboratory · Laboratory of Digital Microscopic Analysis of the Institute of Regenerative Medicine of the Scientific and Technological Park of Biomedicine · Russian Federation · Moscow

Petr Timashev

Academic degree: doctor of chemical sciences

Science rang: docent

First Moscow State Medical University named after I.M. Sechenov scientific adviser · Scientific and Technological Park of Biomedicine · Russian Federation · Moscow

Elena Sadchkova

Academic degree: candidate of chemical sciences

Institute of Gene Biology of the Russian Academy of Sciences Deputy Director for Innovation Work · Russian Federation · Москва

ABSTRACT

Subject. The subject of the study is the biomechanical properties of a personalized collagen membrane in the area of wound defects of the oral mucosa. Objectives. To increase the effectiveness of treatment of patients with extensive surgical wound defects of the oral mucosa by experimentally substantiating the use of developed collagen membranes from bovine Achilles tendon. Methodology. Based on the Central Vivarium of the First Moscow State Medical University named after I.M. Sechenov (Sechenov University) conducted an experimental study on laboratory animals (rabbits) (n = 12) to create a wound surface in the oral cavity (limited vestibuloplasty according to Clark on the upper jaw) and its closure with collagen membranes with an assessment of the course of the postoperative period and subsequent histological examination of biomaterials: 1st – using a personalized collagen membrane, 2nd – using a collagen membrane with lactoferrin, 3rd – using a Mucograft membrane, 4th group – control. Results. In the postoperative period, on the 3rd day, moderate swelling, and hyperemia in the area of vestibuloplasty were noted in the oral cavity of rabbits: the least pronounced swelling was in animals in the 2nd group of operations (1.5 ± 0.5 points) (p = 0.001), hyperemia – in the 3rd group (1.7 ± 0.4 points) (p = 0.001). On the 5th day in the oral cavity of experimental animals, hyperemia of the mucous membrane of the oral vestibule in the 2nd group was 0.8 ± 0.4 points (p = 0.001), and in the 3rd group – 1.8 ± 0.4 points (p = 0.001). According to the histological research method, a significant difference was noted in the quantity and quality of the formed soft tissues in the area of application of collagen matrices compared to healing by secondary intention (p < 0.05). The results of histological examination showed in group 2 epithelization with acanthosis and proliferation of the basal layer. Conclusion. The effectiveness of the developed collagen membranes from the Achilles tendon has been proven in eliminating extensive wound defects of the oral mucosa.

KEYWORDS

personalized collagen membrane, bovine Achilles tendon, wound defect of the oral mucosa, biomechanical properties of the membrane, histological examination

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GOST

Gostev M. S., Tarasenko S. V., Kazumyan S. V., Diachkova E. Y., Usanova A. P., Fayzullin A. L., Timashev P. S., Sadchkova E. R. EXPERIMENTAL RATIONALE FOR THE APPLICATION OF BIORESORBABLE PERSONALIZED COLLAGEN MEMBRANES FOR CLOSURE OF DEFECTS IN THE ORAL MUCOSA // Actual problems in dentistry. 2023. no. 4. pp. 77-82. DOI: https://doi.org/10.18481/2077-7566-2023-19-4-77-82. (Date of access 01.10.2026).

MLA

Mikhail Gostev, Tarasenko S. V., Kazumyan S. V., Diachkova E. Y., Usanova A. P., Fayzullin A. L., Timashev P. S., Sadchkova E. R.. "EXPERIMENTAL RATIONALE FOR THE APPLICATION OF BIORESORBABLE PERSONALIZED COLLAGEN MEMBRANES FOR CLOSURE OF DEFECTS IN THE ORAL MUCOSA." Actual problems in dentistry 19.4 (2023): 77-82. DOI: https://doi.org/10.18481/2077-7566-2023-19-4-77-82.

APA

Gostev M. S., Tarasenko S. V., Kazumyan S. V., Diachkova E. Y., Usanova A. P., Fayzullin A. L., Timashev P. S., Sadchkova E. R. (2023). EXPERIMENTAL RATIONALE FOR THE APPLICATION OF BIORESORBABLE PERSONALIZED COLLAGEN MEMBRANES FOR CLOSURE OF DEFECTS IN THE ORAL MUCOSA. Actual problems in dentistry, 19(4), 77-82. https://doi.org/10.18481/2077-7566-2023-19-4-77-82

Chicago

Mikhail Gostev, Tarasenko S. V., Kazumyan S. V., Diachkova E. Y., Usanova A. P., Fayzullin A. L., Timashev P. S., Sadchkova E. R.. "EXPERIMENTAL RATIONALE FOR THE APPLICATION OF BIORESORBABLE PERSONALIZED COLLAGEN MEMBRANES FOR CLOSURE OF DEFECTS IN THE ORAL MUCOSA." Actual problems in dentistry 19, no. 4 (2023): 77-82. https://doi.org/10.18481/2077-7566-2023-19-4-77-82.

WIKI

{{cite journal | author = Gostev M. S., Tarasenko S. V., Kazumyan S. V., Diachkova E. Y., Usanova A. P., Fayzullin A. L., Timashev P. S., Sadchkova E. R. | title = EXPERIMENTAL RATIONALE FOR THE APPLICATION OF BIORESORBABLE PERSONALIZED COLLAGEN MEMBRANES FOR CLOSURE OF DEFECTS IN THE ORAL MUCOSA | journal = Actual problems in dentistry | year = 2023 | volume = 19 | issue = 4 | pages = 77-82 | doi = 10.18481/2077-7566-2023-19-4-77-82 }}

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EXPERIMENTAL RATIONALE FOR THE APPLICATION OF BIORESORBABLE PERSONALIZED COLLAGEN MEMBRANES FOR CLOSURE OF DEFECTS IN THE ORAL MUCOSA

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