employee
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
UDC 616.31
Bone morphogenetic protein BMP-2 is widely used in bone replacement materials due to its pronounced osteoinductive properties. However, its possible side effects, including the effect on the adhesive activity of pathogenic microorganisms, remain insufficiently studied. The purpose of this work was to evaluate the residual adhesion of microorganisms to the surface of a structural material with applied BMP-2. Materials and methods. In the in vitro study, medical titanium samples with a diameter of 5 mm and a thickness of 0.5 mm were used. Two groups were formed: with BMP-2 applied (experimental) and uncoated (control). The test cultures were E. coli ATCC 25982, S. aureus ATCC 6538, C. albicans CTC885-653, S. mutans 3003, S. mitis NCTC 10712, and P. aeruginosa B-8243. After standard incubation for 40 minutes at 37°C, the samples were treated with ultrasound and applied to nutrient medium. The residual adhesion was estimated by the number of grown colonies with the calculation of the Iad index. Statistical processing was performed using the Shapiro-Wilk test and the Mann–Whitney test (p < 0.05). Results. Residual adhesion of test cultures was noted in all groups. On the surface covered with BMP-2, the highest values of Iad were shown by S. mutans (74.9%) and S. mitis (75%). Minimal adhesion was found for E. coli (11.34%). In the control group, the indicators were similar, there were no statistically significant differences between the groups. However, there was a tendency to decrease adhesion on samples with BMP-2 coating.
secondary adentia, dental implantation, osseointegration, osteoinductive material, modification of the dental implant surface, bacterial adhesion, bone morphogenetic protein 2
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