employee
Ufa, Russian Federation
employee
Moscow, Moscow, Russian Federation
Ufa, Ufa, Russian Federation
Ufa, Ufa, Russian Federation
Ufa, Ufa, Russian Federation
employee from 01.01.2004 until now
Ufa, Ufa, Russian Federation
More than 75% of waste is toxic to the environment and humans. The aim of the study was to invistigate the toxic effect of ecotoxicants on the rudiments of the teeth of laboratory animals. The experiment was carried out on 30 white outbred rats weighing 180–250. All animals were divided into 2 groups: control (Group 1) and experimental (Group 2). During the experiment, all animals of the experimental groups were subjected to inhalation exposure to gasoline and formaldehyde vapors. Animals of the control group were supplied with normal air around the clock. The jaws of rats were decalcified for 30 days in a 10% formic acid solution in a 10% buffered formalin solution. After dehydration of the material in a battery of alcohols of increasing concentration (ethanol - concentration from 70% to 100% absolute) was poured into paraffin according to the generally accepted method. Using a rotary microtome brand LEICA RM 2145 (LEICA, Germany), histological sections 5–8 µm thick were made. The sections were stained with hematoxylin and eosin and picrofuchsin according to the Van Gieson method. Stained sections were examined and photographed using an AXIO IMAGER-Z1 light microscope (CARL ZEISS, Germany). In the control group of rats, a morphological picture of normal tooth histogenesis was observed at a late stage of development. In the structure of the tooth germs of rat pups of the 2nd group, a violation of the processes of histogenesis of most tooth germs and a change in the structure of the periodontal tissues were found. In the epithelium of the gingival mucosa, signs of dystrophic changes were determined, up to cell destruction; in the connective tissue plate of the gingival mucosa, in the periodontium and in the zone of formation of the alveolar bone, signs of inflammatory phenomena were determined in the form of cell infiltrations and circulatory disorders in the form of expansion of the lumens and blood filling of the vessels. In the area of the tooth germs, destructive changes in odontoblasts and enameloblasts were manifested, which was probably one of the reasons for the disruption of the processes of dentinogenesis and enamel formation, expressed in the heterogeneity of the formation of dentin and enamel layers. This confirms the toxic effect of ecotoxicants on the rudiments of the teeth of laboratory animals.
rudiments of teeth, ecotoxicants, morphology, dioxin, formaldehyde
1. Abdazimov A.D. Eksperimental'noe izuchenie deystviya promyshlennyh aerozoley i toksicheskih gazov na sostoyanie zubov. Stomatologiya. 1992;2:6-8. [A.D. Abdazimov. Experimental study of the effect of industrial aerosols and toxic gases on the state of the teeth: Dentistry. 1992;2:6-8. (In Russ.)]. https://translated.turbopages.org/proxy_u/en-ru.ru.fb2a82df-6396c93f-feee4c0b-74722d776562/https/pubmed.ncbi.nlm.nih.gov/1412559/
2. Apraksina E.Yu., Zalavina S.V., Zhleznyy P.A., Zhelezanya A.P. Struktura zubnyh zachatkov i osobennosti mineral'nogo obmena pri vibracionnom vozdeystvii v eksperimente. Problemy stomatologii. 2018;14(2):121-125. [E.Yu. Apraksina, S.V. Zalavina, P.A. Zhlezny, A.P. Zhelezanya. Structure of tooth rudiments and mineral metabolism pattern at vibration effectin the experiment. Actual Problems in Dentistry. 2018;14(2):121-125. (In Russ.)]. https://doi.org/10.18481/2077-7566-2018-14-2-121-125
3. Bazhenova L.N. Organicheskie superekotoksikanty. Analiticheskiy aspekt. Kurs lekciy. Ekaterinburg. 2007:261. [L.N. Bazhenova. Organic superecotoxicants. Analytical aspect. Lecture course. Yekaterinburg. 2007:261. (In Russ.)]. http://hdl.handle.net/10995/1381
4. Rybak V.A. Issledovanie vliyaniya kachestva okruzhayuschey sredy na zdorov'e naseleniya. Moskva. 2014:424. [V.A. Rybak. Study of the impact of environmental quality on public health. Moscow. 2014:424. (In Russ.)]. https://www.logobook.ru/prod_show.php?object_uid=12551440
5. Zaharov A.B. Dendroindikaciya okruzhayuschey sredy promyshlennyh gorodov. Moskva. 2014:144. [A.B. Zakharov. Dendroindication of the environment of industrial cities. Moscow. 2014:144. (In Russ.)]. https://www.logobook.ru/prod_show.php?object_uid=12547866
6. Zul'karnaeva A.T., Chuykin S.V., Aver'yanov S.V., Zul'karnaev T.R. Immunogistohimicheskie issledovaniya vliyaniya ekotoksikantov na zubochelyustnuyu sistemu laboratornyh zhivotnyh. Medicinskiy vestnik Bashkortostana. 2010;4:140-144. [A.T. Zulkarnaeva, S.V. Chuikin, S.V. Averyanov, T.R. Zulkarnaev. Immunohistochemical studies of the effect of ecotoxicants on the dentoalveolar system of laboratory animals. Medical Bulletin of Bashkortostan. 2010;4:140-144. (In Russ.)]. https://elibrary.ru/item.asp?id=15186743
7. Kazubovskaya M.A. Biomonitoring okruzhayuschey sredy. Moskva. 2013:532. [M.A. Kazubovskaya. Biomonitoring of the environment. Moscow. 2013:532. (In Russ.)]. https://www.logobook.ru/prod_show.php?object_uid=12559436
8. Kamilov H.P., Taylakova D.I., Nikol'skaya I.A. Embrional'nyy i postnatal'nyy gistogenez zubov u krys v usloviyah zagryazneniya okruzhayuschey sredy. Medical Journal of the Russian Federation, Russian Journal. 2019;25(4). [Kh.P. Kamilov, D.I. Tailakova, I.A. Nikolskaya. Embryonic and postnatal histogenesis of teeth in rats under environmental pollution. Medical Journal of the Russian Federation, Russian Journal. 2019;25(4). (In Russ.)]. http://dx.doi.org/10.18821/0869-2106-2019-25-4-230-233
9. Kozicyn A.N., Rudoy G.N. Opyt i perspektivy resheniya voprosov ohrany zdorov'ya rabochih i naseleniya, prozhivayuschego na territoriyah razmescheniya promyshlennyh predpriyatiy. Medicina truda i promyshlennaya ekologiya. 2007;3:5-8. [A.N. Kozitsyn, G.N. Rudoy. Experience and prospects for solving issues of protecting the health of workers and the population living in the territories where industrial enterprises are located. Occupational medicine and industrial ecology. 2007;3:5-8. (In Russ.)]. https://cyberleninka.ru/article/n/opyt-i-perspektivy-resheniya-voprosov-ohrany-zdorovya-rabochih-i-naseleniya-prozhivayuschego-na-territoriyah-razmescheniya
10. Kontorschikova K.N. Perekisnoe okislenie lipidov v norme i patologii. Uchebnoe posobie. N. Novgorod. 2000:23. [K.N. Kontorshchikova. Lipid peroxidation in health and disease. Textbook. N. Novgorod. 2000:23. (In Russ.)]. https://cyberleninka.ru/article/n/osobennosti-protsessa-perekisnogo-okisleniya-lipidov-v-norme-i-pri-nekotoryh-patologicheskih-sostoyaniyah-u-detey-obzor-literatury.
11. Mel'nichenko E.M., Cheshko N.N., Berlov G.A. Morfometriya izmeneniy odontogeneza u krys, vyzvannyh malymi dozami ioniziruyuschey radiacii. Zdravoohranenie. 1997;10:19-21. [E.M. Melnichenko, N.N. Cheshko, G.A. Berlov. Morphometry of changes in odontogenesis in rats caused by low doses of ionizing radiation. Healthcare. 1997;10:19-21. (In Russ.)]. http://webirbis.spsl.nsc.ru/irbis64r_01/cgi/cgiirbis_64.exe?I21DBN=MD&P21DBN=MD&S21STN=1&S21FMT=fullwebr&C21COM=S&S21CNR=&S21P01=0&S21P02=1&S21P03=A=&S21STR=%D0%9C%D0%B5%D0%BB%D1%8C%D0%BD%D0%B8%D1%87%D0%B5%D0%BD%D0%BA%D0%BE%2C%20%D0%AD.%20%D0%9C.
12. Olesova V.N., Havkina E.Yu., Soltar' V.I. Effektivnost' stomatologicheskoy dispanserizacii i ezhegodnoy sankcii polosti rta v organizovannyh kollektivah rabotnikov promyshlennyh predpriyatiy. Rossiyskiy stomatologicheskiy zhurnal. 2008;4:46-48. [V.N. Olesova, E.Yu. Khavkina, V.I. Soltar. Efficiency of dental medical examination and annual sanction of the oral cavity in organized collectives of workers of industrial enterprises. Russian Dental Journal. 2008;4:46-48. (In Russ.)]. http://elib.fesmu.ru/elib/Article.aspx?id=188951
13. Panfilova V.V., Kolganova O.I., Chibisova O.F. Vliyanie hronicheskogo elektromagnitnogo oblucheniya na embriogenez i rannee postanal'noe razvitie potomstva obluchennyh zhivotnyh. Radiaciya i risk (Byulleten' Nacional'nogo radiacionno-epidemiologicheskogo registra). 2021;30(4):61-68. [V.V. Panfilova, O.I. Kolganova, O.F. Chibisova. Effect of chronic electromagnetic radiation on embryogenesis and early postnatal development of the offspring of irradiated animals. Radiation and Risk (Bulletin of the National Radiation and Epidemiological Register). 2021;30(4):61-68. (In Russ.)]. https://doi.org/10.21870/0131-3878-2021-30-4-61-68
14. Sklyanov Yu.I., Kolesnikov S.I., Zheleznyy P.A. Vliyanie vibracii na sistemu "mat'-plod" v eksperimente. Moskva. 2014:192. [Yu.I. Sklyanov, S.I. Kolesnikov, P.A. Zhelezny. Influence of vibration on the "mother-fetus" system in the experiment. Moscow. 2014:192. (In Russ.)]. https://www.journal-irioh.ru/jour/article/view/25/0
15. Soltaeva A.M.-H., Dzhambetova P.M., Rubanovich A.V., Genotoksicheskie posledstviya neftyanogo zagryazneniya okruzhayuschey sredy. Moskva. 2014:379. [A.M.-Kh. Soltaeva, P.M. Dzhambetova, A.V. Rubanovich. Genotoxic consequences of oil pollution of the environment. Moscow. 2014:379. (In Russ.)]. https://znanium.com/catalog/document?id=352298
16. Tunakova Yu.A., Novikova S.V., Fayzullin R.I., Valiev V.S. Zavisimosti soderzhaniya mikroelementov vo vnutrennih sredah organizma cheloveka ot soderzhaniya v ob'ektah okruzhayuschey sredy. Rossiyskiy zhurnal prikladnoy ekologii. 2016;4(8):29-34. [Yu.A. Tunakova, S.V. Novikova, R.I. Faizullin, V.S. Valiev. Dependences of the content of microelements in the internal environments of the human body on the content in environmental objects. Russian Journal of Applied Ecology. 2016;4(8):29-34. (In Russ.)]. https://cyberleninka.ru/article/n/zavisimosti-soderzhaniya-mikroelementov-vo-vnutrennih-sredah-organizma-cheloveka-ot-soderzhaniya-v-obektah-okruzhayuschey-sredy
17. Cheshko N.N., Berlov G.A. Izmeneniya zachatka zubov v rannem postnatal'nom periode u krys pod vliyaniem doz ioniziruyuschey radiacii. Sovremennaya stomatologiya. 2000;1:34-36. [N.N. Cheshko, G.A. Berlov. Changes in the rudiment of teeth in the early postnatal period in rats under the influence of doses of ionizing radiation. Modern dentistry. 2000;1:34-36. (In Russ.)]. https://www.mednovosti.by/Journal.aspx?id=173
18. Chuykin S.V., Galeev R.V., Galeeva R.R. Stomatologicheskiy status detey s autizmom, prozhivayuschih v regione s ekotoksikantami. Problemy stomatologii. 2019;2:84-88. [S.V. Chuikin, R.V. Galeev, R.R. Galeeva. Dental status of children with autism living in a region with ecotoxicants. Actual problems in dentistry. 2019;2:84-88. (In Russ.)]. https://cyberleninka.ru/article/n/stomatologicheskiy-status-detey-s-autizmom-prozhivayuschih-v-regione-s-ekotoksikantami
19. Chuykin S.V., Davletshin N.A., Viktorova T.V., Shayhutdinova D.I. Etiologiya, patogenez, klinika i profilaktika vrozhdennoy rasscheliny verhney guby i neba. Ufa. 2007:141. [S.V. Chuikin, N.A. Davletshin, T.V. Viktorova, D.I. Shaikhutdinova. Etiology, pathogenesis, clinic and prevention of congenital cleft lip and palate. Ufa. 2007:141. (In Russ.)]. https://american-issue.info/wp-content/uploads/2019/12/11-14.pdf
20. Chuykin S.V., Bilak A.G., Davletshin N.A. Rasprostranennost', klinikoanatomicheskie formy vrozhdennoy rasscheliny verhney guby, neba i soputstvuyuschie zabolevaniya u detey s dannoy patologiey. Stomatologiya detskogo vozrasta i profilaktika. 2015;3(54):17-21. [S.V. Chuikin, A.G. Bilak, N.A. Davletshin. Prevalence, clinical and anatomical forms of congenital cleft lip, palate and concomitant diseases in children with this pathology. Dentistry of childhood and prevention. 2015;3(54):17-21. (In Russ.)]. https://www.elibrary.ru/item.asp?id=24395018
21. Alaluusua S., Lukinmaa P.L. Developmental dental toxicity of dioxin and related compounds - a review // Int Dent J. - 2006;56(6):323-331. https://pubmed.ncbi.nlm.nih.gov/17243464/
22. Buyse M.L., Birth defect encyclopedia. Dover : Black-well Scientic Publications. 1990:605. https://www.amazon.com/Birth-Defects-Encyclopaedia-Louise-Buyse/dp/0865420882
23. Chlapowska J., Opydo-Szymaczer J. Dietary and hygienic aspects of fluoride exposure in pregnant women // Ann Acad Med Stetin. - 2004;50(l1):19-22. https://pubmed.ncbi.nlm.nih.gov/16892578/
24. Chuykin S., Galeyev R., Galeeva R. Dental status of children with autism, living in the region with ecotoxicants // Actual problems in dentistry. - 2019;9;15(2):84-88. http://dx.doi.org/10.18481/2077-7566-2019-15-2-84-88
25. Hursthouse A., Kowalczyk G. Transport and dynamics of toxic pollutants in the natural environment and their effect on human health: research gaps and challenge // Environ Geochem Health. - 2009;31(2):165-187. https://pubmed.ncbi.nlm.nih.gov/19002593/
26. Jedeon K., De la Dure-Molla M., Brookes S.J., Loiodice S., Marciano C., Kirkham J. et al. Enamel defects reflect perinatal exposure to bisphenol A.Enamel defects reflect perinatal exposure to bisphenol A // Am J Pathol. - 2013;183(1):108-118. https://pubmed.ncbi.nlm.nih.gov/23764278/
27. Khludova L. Molecular mechanisms of action of ecotoxicants on simple nervous systems. neuroscience for medicine and psychology. - 2022;24. http://dx.doi.org/10.29003/m2984.sudak.ns2022-18/368-369
28. Panfilova V., Kolganova O., Chibisova O. The effect of proton and gamma irradiation on the behavior, learning ability and pregnancy in rats. Book of Abstracts. 2021. https://doi.org/10.21175/rad.abstr.book.2021.32.2
29. Romero A.N., Herlin M., Finnilä M., Korkalainen M., Håkansson H., Viluksela M., Sholts SB. Skeletal and dental effects on rats following in utero/lactational exposure to the non-dioxin-like polychlorinated biphenyl PCB 180 // PLoS One. - 2017;12(9):185-241. doi: 10.1371. https://pubmed.ncbi.nlm.nih.gov/28957439/
30. Rossignol D.A., Genuis S.J., Frye R.E. Environmental toxicants and autism spectrum disorders: a systematic review // Transl Psychiatry. - 2014;4:360. https://pubmed.ncbi.nlm.nih.gov/24518398/
31. Wigle D.T., Arbuckle T.E., Turner M.C., Bérubé A., Yang Q., Liu S., Krewski D. Epidemiologic evidence of relationships between reproductive and child health outcomes and environmental chemical contaminants // J Toxicol Environ Health B Crit Rev. - 2008;11(5-6): 373-517. https://pubmed.ncbi.nlm.nih.gov/18470797/
