employee
Ekaterinburg, Ekaterinburg, Russian Federation
Ekaterinburg, Ekaterinburg, Russian Federation
employee
Ekaterinburg, Ekaterinburg, Russian Federation
employee
Ekaterinburg, Russian Federation
employee
Ekaterinburg, Ekaterinburg, Russian Federation
Subject. With increasing age of patients, in many cases, the severity of periodontal diseases also increases, and dystrophic ones join the inflammatory processes. Local predisposing factors in the oral cavity are aggravated by concomitant somatic diseases and a decrease in compensatory processes against the background of physiological aging. Literature data indicate that the manifestations of the physiological process of aging and pathological processes (inflammatory and dystrophic) can be clearly observed on the example of buccal epithelial cells. The aim is to identify the relationship between the age of patients, their periodontal status and the cytological characteristics of buccal epithelial cells. Methodology. All study participants were divided into two groups: the first included patients with a conditionally healthy periodontium (72 people), the second - with a chronic inflammatory process in the periodontium (57 people). In accordance with the WHO classification, a gradation of age was carried out: young (18―44 years old), mature (45―59 years old), elderly (60―74 years old), senile (75―90 years old). All patients underwent a comprehensive dental examination, sampling and cytological examination of the buccal epithelium, calculation of the integral indices of the buccal cytogram. Results. In healthy patients, a weak positive correlation was established between the cytogenetic index, the index of the accumulation of cytogenetic disorders and age, a weak negative correlation was found between the proliferative index and age. In patients with an inflammatory process in the periodontal tissues, a multidirectional change in the values of the buccal cytogram indices was observed, which may indicate an imbalance in the processes of regeneration, apoptosis, and cellular damage in inflammatory periodontal diseases in older age groups. Conclusions. In patients with healthy periodontal disease, a regular accumulation of cytogenetic disorders occurs with increasing age, while proliferative activity, on the contrary, decreases. Patients with inflammatory phenomena in the periodontal tissues are characterized by imbalance, impaired coordination of regeneration and apoptosis processes, combined with the most pronounced reactivity in the middle age period.
aging, biological age, degenerative-dystrophic changes, periodontal disease, cytological examination, buccal epithelium, buccal cytogram
1. Bazarnyy, V. V., Polushina, L. G., Maksimova, A. Yu, Svetlakova, E. N., Sementsova, E. A., Nersesyan, P. M., Mandra, J. V. (2019). Ispolzovaniye integralnykh indeksov v otsenke bukkalnoy tsitogrammy v norme i pri patologii polosti rta [The use of integral indices in the assessment of the buccal cytogram in health and disease of the oral cavity]. Klinicheskaya laboratornaya diagnostika [Clinical laboratory diagnostics], 64 (12), 736-740. (In Russ.)
2. Bazarnyy, V. V., Polushina, L. G., Maksimova, A. Yu., Svetlakova, E. N., Mandra, J. V. (2018). Tsitologicheskaya kharakteristika bukkalnogo epiteliya pri khronicheskom generalizovannom parodontite [Cytological characteristics of buccal epithelium in chronic generalized periodontitis]. Klinicheskaya laboratornaya diagnostika [Clinical laboratory diagnostics], 12, 773-776. (In Russ.)
3. Bazarnyy, V. V., Polushina, L. G., Maksimova, A. Yu., Svetlakova, E. N., Mandra, J. V. (2018). Patogeneticheskoye obosnovaniye novykh podkhodov k otsenke sostoyaniya tkaney polosti rta pri khronicheskom generalizovannom parodontite [Pathogenetic substantiation of new approaches to assessing the state of oral tissues in chronic generalized periodontitis]. Problemy stomatologii [Actual Problems in dentistry], 14 (4), 14-18. (In Russ.)
4. Deryugina, A. V., Ivashchenko, M. N., Ignatyev, P. S. et al. (2018). Otsenka genotoksichnykh effektov v bukkalnom epitelii pri narusheniyakh adaptatsionnogo statusa organizma [Evaluation of genotoxic effects in the buccal epithelium in violation of the adaptive status of the organism]. Klinicheskaya laboratornaya diagnostika [Clinical laboratory diagnostics], 63 (5), 290-292. (In Russ.)
5. Kolosnitsyna, M., Khorkina, N. (2016). Gosudarstvennaya politika aktivnogo dolgoletiya: o chem svidetelstvuyet mirovoy opyt [State policy of active longevity: as evidenced by world experience]. Demograficheskoye obozreniye [Demographic Review], 3 (4), 27-46. (In Russ.)
6. Mandra, J. V., Bazarnyy, V. V., Chupakhin, O. N., Khonina, T. G., Sementsova, E. A., Svetlakova, E. N., Kotikova, A. Yu., Legkikh, A. V., Polushina, L. G., Teslenko, A. Yu. (2017). Kliniko-morfologicheskaya otsenka effektivnosti primeneniya innovatsionnoy lechebno-profilakticheskoy zubnoy pasty v kompleksnom lechenii patsiyentov molodogo vozrasta s osnovnymi stomatologicheskimi zabolevaniyami [Clinical and morphological assessment of the effectiveness of the use of innovative therapeutic and prophylactic toothpaste in the complex treatment of young patients with major dental diseases]. Problemy stomatologii [Actual problems in dentistry], 13 (3), 29-35. (In Russ.)
7. Mandra, J. V., Kaminskaya, L. A., Svetlakova, E. N., Gavrilov, I. V., Zholondziovskiy, P. A., Timerbulatov, A. D. (2016). Dinamika izmeneniya biokhimicheskogo sostava slyuny pod vliyaniyem uglevodsoderzhashchikh produktov “legkogo pitatsiya” [Dynamics of changes in the biochemical composition of saliva under the influence of carbohydrate-containing products "light nutrition"]. Problemy stomatologii [Actual problems in dentistry], 12 (4), 10-16. (In Russ.)
8. Nersesyan, P. M., Zholudev, S. E., Polushina, L. G., Maksimova, A. Yu., Bazarnyy, V. V. (2019). Laboratornoye obosnovaniye atravmatichnosti ispolzovaniya individualnogo formirovatelya desny pri dentalnoy implantatsii [Laboratory substantiation of the atraumatic nature of the use of an individual gum shaper for dental implantation]. Uralskiy meditsinskiy zhurnal [Ural Medical Journal], 177 (9), 37-40. (In Russ.)
9. Sedov, E. V., Linkova, N. S., Kozlov, K. L., Kvetnaya, T. V., Konovalov, S. S. (2013). Bukkalnyy epiteliy kak obyekt otsenki biologicheskogo vozrasta i tempa stareniya organizma [Buccal epithelium as an object of assessing the biological age and rate of aging of the body]. Uspekhi gerontologii [Advances in gerontology], 26 (4), 610-613. (In Russ.)
10. Arul, P., Shetty, S., Masilamani, S. (2017). Evaluation of micronucleus in exfoliated buccal epithelial cells using liquid-based cytology preparation in petrol station workers. Indian J. Med Paediatr Oncol, 38 (3), 273-276.
11. Benvindo-Souza, M., Assis, R. A., Oliveira, E. A., Borges, R. E., Santos, L. R. (2017). The micronucleus test for the oral mucosa: global trends and new questions. Environ Sci Pollut Res Int., 24 (36), 27724-27730.
12. Cuello-Almarales, D. A., Almaguer-Mederos, L. E. (2017). Buccal cell micronucleus frequency is significantly elevated in patients with spinocerebellar ataxia type 2. Archives of Medical Research, 48 (3), 297-302.
13. erici Eker, E., Koyuncu, H. (2016). Determination of genotoxic effects of hookah smoking by micronucleus and chromosome aberration methods. Med Sci Monit., 21, 4490-4494.
14. Fougère, B., Boulanger, E. (2017). Chronic inflammation: accelerator of biological aging. J Gerontol A Biol Sci Med Sci., 72 (9), 1218-1225.
15. François, M., Leifert, W. (2014). Altered cytological parameters in buccal cells from individuals with mild cognitive impairment and Alzheimer's disease. Cytometry, 85 (8), 698-708.
16. Gajaria, P. K., Maheshwari, U. M. (2017). Buccal mucosa exfoliative cell prussian blue stain co-relates with iron overload in β-thalassemia major patients. Indian J Hematol Blood Transfus, 33 (4), 559-564.
17. Gómez-Meda, B. C., Ramírez-Aguilar, M. Á., Zúñiga-González, G. M. (2015). Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases. Journal Periodontal Research, 50 (1), 28-36.
18. Hopf, N. B., Danuser, B., Bolognesi, C., Wild, P. (2020). Age related micronuclei frequency ranges in buccal and nasal cells in a healthy population. Environ Res, 180. doi:https://doi.org/10.1016/j.envres.2019.108824.
19. Khan, S., Khan, A. U., Hasan, S. (2016). Genotoxic assessment of chlorhexidine mouthwash on exfoliated buccal epithelial cells in chronic gingivitis patients. Journal Indian Society Periodontology, 20 (6), 584-591.
20. Nallamala, S., Guttikonda, V. R., Manchikatla, P. K., Taneeru, S. (2017). Age estimation using exfoliative cytology and radiovisiography: A comparative study. J Forensic Dent Sci., 9 (3), 144-148.
21. Petrashova, D. A. (2019). Buccal epitelium cytogenetic status in schoolchildren living in high and middle latitudes. Klin Lab Diagn., 64 (4), 229-233. doi:https://doi.org/10.18821/0869-2084-2019-64-4-229-233.
22. Saba, R., Halytskyy, O., Saleem, N., Oliff, I. A. (2017). Buccal epithelium, cigarette smoking, and lung cancer. Review of the Literature. J.Oncology, 93, 347-353.
23. Sahu, M., Suryawanshi, H., Nayak, S., Kumar, P. Cytomorphometric analysis of gingival epithelium and buccal mucosa cells in type 2 diabetes mellitus patients. Journal Oral and Maxillofacial Pathology, 21 (2), 224-228.
24. Shashikala, R., Indira, A. P., Manjunath, G. S. (2015). Role of micronucleus in oral exfoliative cytology. J Pharm Bioallied Sci., 409-413.
25. Shetty, D. C., Wadhwan, V., Khanna, K. S., Jain, A., Gupta, A. (2015). Exfoliative cytology: A possible tool in age estimation in forensic odontology. J Forensic Dent Sci., 7 (1), 63-66.
26. Upadhyay, M., Verma, P., Sabharwal, R., Subudhi, S. K., Jatol-Tekade, S., Naphade, V., Choudhury, B. K., Sahoo, P. D. (2019). Micronuclei in Exfoliated Cells: A Biomarker of Genotoxicity in Tobacco Users. Niger J Surg, 25 (1), 52-59.
27. Wael Youssef, E. (2018). Age-Dependent Differential Expression of Apoptotic Markers in Rat Oral Mucosa. Asian Pac J Cancer Prev., 19 (11), 3245-3250.
28. Zamora-Perez, A. L., Ortiz-García, Y. M., Lazalde-Ramos, B. P. (2015). Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases. J Periodontal Res, 50 (1), 28-36.