employee
Ufa, Ufa, Russian Federation
employee
Perm', Perm, Russian Federation
employee
Ufa, Ufa, Russian Federation
employee
Ufa, Ufa, Russian Federation
employee
Ufa, Ufa, Russian Federation
employee
Ufa, Ufa, Russian Federation
Ufa, Ufa, Russian Federation
Subject. The antagonistic activity of the Bacillus Subtilis 3H probiotic was studied on a vast amount of data, which makes it possible to rationalize its use in a periodontal practice. The aim of the paper is to substantiate the use of the Bacillus Subtilis 3H probiotic in periodontal practice by studying the antagonistic activity against periodontal-pathogenic microflora and yeast-like fungi of Candida species. Material and methods. The Bacillus Subtilis 3H probiotic sensitivity of reference (archival) strains, clinical isolates from patients with generalized chronic periodontitis associated with yeast-like Candida species fungi, as well as bacteria test cultures and clinical strains of periodontal pathogens, were studied using the deferred antagonism method (diffusion method, perpendicular streak technique). Results. The conducted study made it possible to establish sufficient antagonistic activity of Bacillus Subtilis 3H probiotic against reference strains, clinical isolates isolated from patients with chronic generalized periodontitis associated with yeast-like Candida species fungi, as well as bacteria test cultures and clinical strains of periodontal pathogens. Conclusions. The study findings allow us to recommend the Bacillus Subtilis 3H probiotic to be widely used in the complex treatment of generalized chronic periodontitis caused by periodontopathic microflora, as well as by yeast-like Candida species fungi.
generalized chronic periodontitis, yeast-like Candida species fungi, probiotics, Bacillus Subtilis bacteria, antagonistic activity, reference strains, clinical isolates
1. Dzyuba, E. V., Nagaeva, M. O., Zhdanova, E. V. (2019). Rol` immunologicheskix processov v razvitii vospalitel`ny`x zabolevanij parodonta i vozmozhnosti ix korrekcii [The role of immunological processes in the development of inflammatory periodontal diseases and the possibility of their correction]. Problemy` stomatologii [Actual problems in dentistry], 15, 2, 25-31. (In Russ.)
2. Mirsaeva, F. Z. Osobennosti patogeneza hronicheskogo generalizovannogo parodontita u bol'nyh allergicheskimi zabolevaniyami lekarstvennogo geneza / F. Z. Mirsaeva, F. Spadari, G. F. Ziganshina // Novoe v stomatologii. - 2009. - № 7. - S. 25-26.
3. Melexov, S. V. (2009). Rol` drozhzhepodobny`x gribov roda Candida v razvitii patologii parodonta [Role of yeast-like fungi of the genus Candida in the development of periodontal pathology]. Mae`stro [Maestro], 2, 72-75. (In Russ.)
4. Grudyanov, A. I., Fomenko, E. V. (2010). E`tiologiya i patogenez vospalitel`ny`x zabolevanij parodonta [Etiology and pathogenesis of inflammatory periodontal diseases]. Moscow : MIA, 90. (In Russ.)
5. Puzin, N. M., Kiparisova, E. S., Vagner, V. D. et al. (2010). E`tiopatogeneticheskie aspekty` xronicheskogo generalizovannogo parodontita [Etiopathogenetic aspects of chronic generalized periodontitis]. Rossijskij stomatologicheskij zhurnal [Russian Dental Journal], 1, 28-33. (In Russ.)
6. Artushkevich, V. G. (2010). Vliyanie sistemnogo osteoporoza na razvitie generalizovannogo parodontita [The influence of systemic osteoporosis on the development of generalized periodontitis]. Lechenie i profilaktika [Treatment and prevention], 2 (3), 42-47. (In Russ.)
7. Bulkina, N. V., Vedyaeva, A. P. (2012). K voprosu e`tiologii i patogeneze by`stroprogressiruyushhego parodontita [On the etiology and pathogenesis of rapidly progressive periodontitis]. Rossijskij stomatologicheskij zhurnal [Russian Dental Journal], 5, 50-52. (In Russ.)
8. Shirokov, V. Yu., Zhdanov, O. Yu., Ivanov, A. N. (2015). Izmeneniya adgezionnoj funkcii e`ndoteliya i citokinovy`j balans u bol`ny`x xronicheskim generalizovanny`m parodontitom [Changes in the adhesion function of the endothelium and the cytokine balance in patients with chronic generalized periodontitis]. Parodontologiya [Periodontology], 2, 75, 55-59. (In Russ.)
9. Uspenskaya, O. A., Kachesova, E. S. (2017). Rol` obshhix i mestny`x faktorov v vozniknovenii i razvitii xronicheskogo generalizovannogo parodontita tyazheloj stepeni [The role of general and local factors in the emergence and development of severe chronic generalized periodontitis]. Sovremenny`e problemy` nauki i obrazovaniya [Modern problems of science and education], 5. 188. (In Russ.)
10. Rams, T. E., Slots, J. (2014). Candida biotypes in human adult periodontitis. J. Oral. Microbiol, 6, 3, 191-192.
11. Waltimo, T. M., Siren, E. K., Torkko, H. L., Olsen, I., Haapasalo, M. P. (2017). Fungi in therapy-resistant apical periodontitis. Int. Endod. J, 30, 2, 96-101.
12. Grudyanov, A. I., Fomenko, E. V. (2010). E`tiologiya i patogenez vospalitel`ny`x zabolevanij parodonta [Etiology and pathogenesis of inflammatory periodontal diseases]. Moscow : MIA, 90. (In Russ.)
13. Chepurkova, O. A. (2008). Vy`bor antimikrobny`x, antisepticheskix i antimikoticheskix preparatov v kompleksnoj terapii xronicheskogo generalizovannogo parodontita, associirovannogo s drozhzhevy`mi gribami roda Candida [The choice of antimicrobial, antiseptic and antimycotic drugs in the treatment of chronic generalized periodontitis associated with yeast fungi of the genus Candida]. Rossijskij stomatologicheskij zhurnal [Russian Dental Journal], 6, 48-51. (In Russ.)
14. Bejbulatov, G. D., Ostrovskaya, L. Yu., Lepilin, A. V. (2014). Faktory`, vliyayushhie na razvitie kandida-associirovannogo parodontita [Factors affecting the development of candida-associated periodontitis]. Rossijskij stomatologicheskij zhurnal [Russian Dental Journal], 4, 36-38. (In Russ.)
15. Subgingival distribution of yeast and their antifungal susceptibility in immunocompetent subjects with and without dental devices / V. M. Jewtuchowicz [et al.] // Acta. Odontol. Latinoam. - 2017. - Vol. 20, № 1. - P. 17-22.
16. Devi, P., Pradeep, A. R. (2009). Classification of periodontal diseases: the dilemma continues. NY State Dent J, 75, 4, 30-34.
17. Moellering, R. C. (2011). Tissue directed antibiotic therapy. Am. J. Med, 91, 3, 1-45.
18. Orexova, L. Yu., Zhavoronkova, M. D., Suborova, T. N. (2013). Sovremenny`e texnologii bakteriologicheskogo issledovaniya parodontal`ny`x prostranstv [Modern technologies of bacteriological research of periodontal spaces]. Parodontologiya [Periodontology], 2 (67), 9-13. (In Russ.)
19. Torabinejad, M., Eby, W. C., Nai-dorf, I. J. (2015). Inflammatory and immunological aspects of the pathogenesis of human chronic periapical lesions. J. Endodond, 11, 6, 479-484.
20. Henderson, D., Poole, S., Wilson, M. (1996). Bacterial modulines: a novel class of virulence factors which cause host tissue pathology by inducing cytokine synthesis. Microbiol Rev, 60 (2), 316-341.
21. Grudyanov, A. I., Dmitrieva, N. A., Fomenko, E. V. (2002). Primenenie tabletirovanny`x form probiotikov Bifidumbakterina i Acilakta v kompleksnom lechenii vospalitel`ny`x zabolevanij parodonta [The use of tablet forms of the probiotic Bifidumbacterin and Acylact in the complex treatment of inflammatory periodontal diseases]. Stomatologiya [Dentistry], 1, 39-43. (In Russ.)
22. Xoruzhaya, R. E., Pedorecz, A. P. (2008). E`ffektivnost` lecheniya vospalitel`ny`x zabolevanij parodontal`nogo kompleksa pri uslovii vklyucheniya v sxemu terapevticheskix vozdejstvij probiotika «Sporobakterina» [The effectiveness of the treatment of inflammatory diseases of the periodontal complex, provided that the probiotic “Sporobacterin” is included in the therapeutic regimen]. Zbіrnik statej [Zbirnik articles], 12, 1, 274-277. (In Ukr.)
23. Chepurkova, O. A., Chesnokova, M. G., Nedoseko, V. B. et al. (2011). Principy` terapii kandida-associirovannogo parodontita [The principles of treatment of candida-associated periodontitis]. Stomatolog [Dentist], 2, 21-27. (In Russ.)
24. Perevoshhikova, O. A. (2013). Primenenie probiotikov v kompleksnom lechenii vospalitel`ny`x zabolevanij parodonta na fone somaticheskoj patologii: avtoref. dis. … kand. med. nauk : 14.01.14 [The use of probiotics in the complex treatment of inflammatory periodontal diseases on the background of somatic pathology: author. dis. ... cand. honey. Sciences: 14.01.14]. Moscow, 19. (In Russ.)
25. Bekirova, L. G., Dzhafarova, A. R., Yusubova, Sh. R. et al. (2019). Povy`shenie e`ffektivnosti kompleksnogo lecheniya vospalitel`ny`x zabolevanij parodonta [Increasing the effectiveness of complex treatment of inflammatory periodontal diseases]. Problemy` stomatologii [Actual problems in dentistry], 15, 4, 84-91. (In Russ.)
26. Redinova, T. L., Zorin, A. Yu., Biktimerova, O. O. et al. (2016). E`ffektivnost` probiotikov v lechenii i profilaktiki stomatologicheskix zabolevanij [The effectiveness of probiotics in the treatment and prevention of dental diseases]. Problemy` stomatologii [[Actual problems in dentistry], 12, 1, 50-56. (In Russ.)
27. Bonifait, L., Chandad, F., Grenier, D. (2009). Probiotics for Orai Heaith: Myth or Reality. JCDA, 75, 8, 585-590. https:// docplayer/net/20903984-Probiotics-for-oral-health-myth-or-reality.html.
28. Sergeev, A. Yu. (2000). Rukovodstvo po laboratornoj diagnostike onixomikozov [Manual for the laboratory diagnosis of onychomycosis]. Moscow : Ge`otar-Media, 160. (In Russ.)
29. Mirsaeva, F. Z., Spadari, F., Ziganshina, G. F. (2009). Osobennosti patogeneza xronicheskogo generalizovannogo parodontita u bol`ny`x allergicheskimi zabolevaniyami lekarstvennogo geneza [Features of the pathogenesis of chronic generalized periodontitis in patients with allergic diseases of medicinal origin]. Novoe v stomatologii [New in dentistry], 7, 25-26. (In Russ.)
30. Jewtuchowicz, V. M. et al. (2017). Subgingival distribution of yeast and their antifungal susceptibility in immunocompetent subjects with and without dental devices. Acta. Odontol. Latinoam, 20, 1, 17-22.
