FEATURES OF THE FUNCTIONAL ACTIVITY OF NEUTROPHILS AND THE LEVEL OF CYTOKINES OF THE LOCAL FOCUS OF INFLAMMATION IN PATIENTS WITH CHRONIC PERIODONTITIS
Abstract and keywords
Abstract (English):
Subject. The risks of an unfavorable outcome of simultaneous implantation in most cases are associated with the installation of an implant in the tooth wells with chronic foci of odontogenic infection. The goal is to study the effector indicators of phagocytes and the level of cytokines of the local focus of inflammation in patients with various forms of chronic periodontitis. Methodology. The study involved 65 dental patients with a diagnosis of "K 04.5―K 04.9" (ICD-10, 1997), a comprehensive examination using laboratory immunological methods which included the study of the functional activity of neutrophils (phagocytic activity and intensity on a model of latex particle absorption; lysosomal activity and index of the total luminescence of lysosomes; HCT-reducing activity and intensity according to spontaneous and induced tests) and the concentration of IL-1-RA IL-1β, IL-2, IL-8, IFN–α, IFN-γ (PCG/ml) wound detachable wells of the extracted tooth. Results. A study of the immunological parameters of the local foci of periapical inflammation in patients with various forms of chronic periodontitis revealed some features of the cytokine profile and the functional activity of neutrophils in the wound detachable hole of the extracted tooth. The data obtained in patients with chronic fibrous periodontitis allowed us to determine the signs of activity of chronic inflammation. In subjects who had their teeth removed due to chronic granulating periodontitis, immunological predictors of a prolonged course of inflammatory and reparative processes were revealed. In patients with chronic granulomatous lesions of the periapical zone, evidence of prolonged contamination of the periapical zone was obtained, which may lead to changes in effector parameters against the background of the preserved bactericidal potential of neutrophilic granulocytes. Findings. Immunological predictors of the development of infectious and inflammatory complications were identified in the context of the planned simultaneous dental implantation when implants are inserted into the wells of the teeth with chronic periodontal foci.

Keywords:
chronic periodontitis, HCT test, local immunity, simultaneous implantation, cytokines
References

1. Aznabaeva, L. F., Arefieva, N. A., Mashko, P. N. et al. (2004). Tsitokiny v immunopatogeneze gnoynogo rinosinusita s zatyazhnym i khronicheskim techeniyem [Cytokines in the immunopathogenesis of purulent rhinosinusitis with a protracted and chronic course]. Rossiyskaya rinologiya [Russian rhinology], 1, 68-71. (In Russ.)

2. Alexandrova, Yu. N. (2007). O sisteme tsitokinov [About the system of cytokines]. Pediatriya [Pediatrics], 86, 3, 124-128. (In Russ.)

3. Bezrukova, A. P. (1999). Parodontologiya [Periodontology]. Moscow, 332. (In Russ.)

4. Bondarenko, I. V., Sysolyatin, S. P., Bondarenko, O. V. (2006). Sovremennyye podkhody k immunoprofilaktike i immunoterapii pri operatsiyakh na litse i chelyustyakh [Present-day approaches to immunoprophylaxis and immunotherapy in maxillofacial surgery]. Rossiyskiy stomatologicheskiy zhurnal [Russian Dental Journal], 6, 7-11. (In Russ.)

5. Dolgushin, I. I., Bukharin, O. V. (2001). Neytrofily i gomeostaz [Neutrophils and homeostasis]. Yekaterinburg, 259. (In Russ.)

6. Dolgushin, I. I. (2002). Rol' neytrofilov v regulyatsii antimikrobnoy rezistentnosti [Role of neutrophils in the regulation of antimicrobial resistance]. Vestnik Rossiyskoy akademii meditsinskikh nauk [Bulletin of the Russian Academy of Medical Sciences], 3, 16-20. (In Russ.)

7. Durnovo, E. A. (2005). Sravnitel'nyy analiz funktsional'noy aktivnosti neytrofilov krovi i rotovoy polosti u bol'nykh s gnoyno vospalitel'nym protsessom v polosti rta [Comparative analysis of the functional activity of blood and oral cavity neutrophils in patients with suppurative inflammatory process in the oral cavity]. Stomatologiya [Dentistry], 3, 29-32. (In Russ.)

8. (2018). Klinicheskiye rekomendatsii (protokoly lecheniya) pri diagnoze bolezni periapikal'nykh tkaney. Utverzhdeny Postanovleniyem № 15 Soveta Assotsiatsii obshchestvennykh ob"yedineniy «Stomatologicheskaya Assotsiatsiya Rossii» ot 30 sentyabrya 2014 goda [Clinical recommendations (treatment protocols) for the diagnosis of periapical tissue disease. Approved by Decree No. 15 of the Council of the Association of Public Associations "Dental Association of Russia" dated September 30, 2014]. (In Russ.)

9. Latyushina, L. S. (2009). Kliniko-immunologicheskaya otsenka effektivnosti lokal'noy immunokorrektsii v kompleksnom lechenii gnoyno-vospalitel'nykh zabolevaniy chelyustno-litsevoy oblasti : avtoref. ... dis. dok. med. nauk [Clinical and immunological evaluation of the effectiveness of local immunocorrection in the complex treatment of purulent-inflammatory diseases of the maxillofacial region : abstract of diss. ... doc. med. sciences]. Chelyabinsk. (In Russ.)

10. Latyushina, L. S., Berezhnaya, E. S., Dolgushin, I. I. et al. (2017). Vliyaniye immunoterapii rekombinantnym IL-1V na kliniko-immunologicheskiye pokazateli patsiyentov s oslozhnennymi perelomami nizhney chelyusti [The effect of immunotherapy with recombinant IL-1β on clinical and immunological parameters of patients with complicated fracture of the mandible]. Problemy stomatologii [Actual problems in dentistry], 13, 2, 49-53. DOI: 10.18481 / 2077-7566-2017-13-2-49-53

11. Latyushina, L. S., Piotrovich, A. V., Dolgushin, I. I. (2016). Vliyaniye lokal'nogo primeneniya rekombinantnykh tsitokinov na techeniye i iskhod dental'noy implantatsii s zakrytym sinus-liftingom [The influence of recombinant cytokines local application on the course and outcome of closed sinus lift dental implantation]. Problemy stomatologii [Actual problems in dentistry], 12, 2, 102-110. DOI: 10.18481 / 2077-7566-2016-12-2-10-10-1-110 (In Russ.)

12. Latyushina, L. S., Piotrovich, A. V., Dolgushin, I. I. et al. (2018). Vliyaniye topicheskoy tsitokinoterapii rekombinantnym IIL-2 (ronkoleykinom) na kliniko-immunologicheskiye parametry patsiyentov pri provedenii zakrytogo sinus-liftinga s odnomomentnoy dental'noy implantatsiyey [Impact of topical cytokine therapy using recombinant il-2 (roncoleukin) on the clinical and immunological parameters of patients received closed sinus-lifting with single-stage dental implantation]. Problemy stomatologii [Actual problems in dentistry], 14, 1, 83-88. DOI: 10.24411 / 2077-7566-2018-000016 (In Russ.)

13. Mayanskiy, A. N., Mayanskiy, N. A., Zaslavskaya, M. A. et al. (1999). Apoptoz neytrofilov [Apoptosis of neutrophils]. Immunologiya [Immunology], 20, 6, 12-19. (In Russ.)

14. Mayanskiy, A. N., Viksman, M. K. (1979). Sposob otsenki funktsional'noy aktivnosti neytrofilov cheloveka po reaktsii vosstanovleniya nitrosinego tetrozoliya : metod, rekomendatsii [Method for assessing the functional activity of human neutrophils by the reaction of nitro blue tetrosolium reduction: method, recommendations]. Kazan', 11. (In Russ.)

15. Mitronin, A. V. (2004). Kompleksnoye lecheniye i reabilitatsiya bol'nykh s destruktivnymi formami khronicheskogo periodontita : avtoreferat diss. ... dok. med. nauk [Complex treatment and rehabilitation of patients with destructive forms of chronic periodontitis : author. diss. ... doc. med. sciences]. Moscow. (In Russ.)

16. Redinova, T. L. (2010). Kariyes i yego oslozhneniya: sootvetstviye nauchnykh otechestvennykh klassifikatsiy i mezhdunarodnoy klassifikatsii bolezney (MKB-10) [Caries and its complications: compliance of domestic scientific classifications and the international classification of diseases (ICD-10)]. Endodontiya Today [Endodontics Today], 1, 37-43. (In Russ.)

17. Sablina, G. I., Kovtonyuk, P. A., Soboleva, N. N. et al. (2011). Sistematika khronicheskikh periodontitov i ikh mesto v MKB-10 [Classification of chronic periodontitis and its position in ICD-10]. Sibirskiy meditsinskiy zhurnal [Siberian Medical Journal], 105, 6, 300-302. (In Russ.)

18. Simbirtsev, A. S. (2002). Tsitokiny - novaya sistema regulyatsii zashchitnykh reaktsiy organizma [Cytokines as a new system, regulating body defense reactions]. Tsitokiny i vospaleniye [Cytokines and inflammation], 1 (1), 9-16. (In Russ.)

19. Totolyan, A. A., Freidlin, I. S. (2000). Kletki immunnoy sistemy [Cells of the immune system]. St. Petersburg : Nauka, 231. (In Russ.)

20. Freydlin, I. S., Totolyan, A. A. (1986). Sposob opredeleniya funktsional'noy aktivnosti fagotsitiruyushchikh kletok [A method for determining the functional activity of phagocytic cells]. SSSR. (In Russ.)

21. Baggiolini, M., Dewald, B., Moser, B. (1997). Human chemokines: an update. Annu.Rev. Immunol, 15, 675-705.

22. Ben Baruch, A., Michiel, D., Oppenheim, J. (1995). Signals and receptors involved in recruitment of inflammatory cells. J. Biol. Chem, 270, 11703-11706.

23. Bidwell, J., Keen, L., Gallageher, G. et al. (1999). Cytokine gene polymorphism in human disease: on-line database. Genes and Immunity, 1, 3-19.

24. Brinkmann, V., Rechard, U., Goosmann, C. et al. (2004). Neutrophil extracelllulartraps kill bacteria. Sciense, 303, 1532-1535.

25. Cantagrel, A., Navaux, F., Loubet-Lescoulie, P. et al. (1999). IL-1beta, IL-1 receptor antagonist, IL-4, and IL-10 gene polymorphism. Arthritis Rheum, 42 (6), 1093-1100.

26. Fernandez Botran, R., Chilton, P., Ma, Y. (1996). Soluble cytokine receptors: their rolesin immunoregulation, disease and therapy. Adv. Immunol, 63, 269-336.

27. Mantovani, A., Bussolino, F., Introna, M. (1997). Cytokine regulation of endothelial cellfunction: from molecular level to the bed side. Immunol. Today, 18, 231-239.

28. Eds. Oppenheim, J., Feldman, M. (2000). Cytokine Reference. London : AcademicPress, 2015.


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