Krasnodar, Krasnodar, Russian Federation
Relevance of the research topic. The oral cavity is one of the most diverse microbiomes in the human body, which is divided into several biotopes: oral mucosa, dental plaque, gingival fluid and gingival sulcus zone and others. The biotope of the gingival sulcus is unique in that quantitative and qualitative changes in the microbial communities of this microecological system can lead to the development of the main dental diseases - gingivitis, periodontitis and caries. The scientific literature on the etiology of these diseases determines the microbial landscape of the gingival sulcus as the dominant causative factor. A significant influence on the microflora of periodontal tissues is exerted by various stressors of a modern person. Purpose ― to study the influence of emotional stress on the microbal landscape of the gingival furrow, which is in a state of eubiosis and dysbiosis. Materials and methods. The study involved 67 aged by 35―44 years. A microbiological study was made of the general microbial contamination of the gingival furrow and its colonization by individual microbal species using aerobic and anaerobic cultivation methods. Results. In the state of relative dormancy, 100 % of people without gum disease were found to have an eubiotic condition of the gingival microbal, 100 % of persons with periodontis - the microbal of the gingival groove were in a state of dysbiosis. Summary. The influence of the stressor on the disorder of the balance of the microbial homeostasis of the dental gingival slit causes an increase in the imbalance of microbial associations in the form of a decrease in the comensal microflora and an increase in the opportunistic pathogenicity.
microbal landscape, emotional stress, periodontis, dysbiosis, eubiosis
1. Gazhva, S. I., Guluyev, R. S. (2012). Rasprostranennost' i intensivnost' vospalitel'nykh zabolevaniy parodonta (obzor literatury) [The prevalence and intensity of inflammatory periodontal diseases (literature review)]. Obozreniye [Review], 1 (75), 13-16. (In Russ.)
2. Colombo, A. P., Bennet, S., Cotton, S. L., Goodson, J. M., Kent, R., Haffajee, A. D., Socransky, S. S., Hasturk, H., Van Dyke, T. E., Dewhirst, F. E., Paster, B. J. (2012). Impact of periodontal therapy on the subgingival microbiota of severe periodontitis: comparison between good responders and individuals with refractory periodontitis using the human oral microbe identification microarray. J Periodontol., 83 (10), 1279-1287. DOIhttps://doi.org/10.1902/jop.2012.110566
3. Stepanova, T. YU., Timofeyeva, A. V. (2016). Mikrobiom rotovoy polosti cheloveka [Microbiome of the human oral cavity]. Sovremennyye problemy nauki i obrazovaniya [Modern problems of science and education], 5, 308. (In Russ.)
4. Markina, T. V., Mayboroda, YU. N., Uryas'yeva, E. V. (2013). Bakterial'nyy spektr slizistoy obolochki organov rta i parodontal'nykh karmanov u patsiyentov s parodontitom [Bacterial spectrum of the mucous membrane of the oral organs and periodontal pockets in patients with periodontitis]. Bacterial spectrum of the mucous membrane of the oral organs and periodontal pockets in patients with periodontitis [Medical Bulletin of the North Caucasus], 1, 45-47. (In Russ.)
5. Bondarenko, V. (2007). Mikroflora cheloveka: norma i patologiya [Microflora of the person: norm and pathology]. Nauka v Rossii [Science in Russia], Moscow, 1 (157). (In Russ.)
6. Bykov, I. M., Ladut'ko, A. A., Esaulenko, E. E., Erichev, I. V. (2008). Biokhimiyarotovoi i desnevoi zhidkosti [Oral and gingival biochemistry]. Krasnodar. (In Russ.)
7. Chertsova, A. I. Otsenka emotsional'nogo napryazheniya studentov vuza [Elektronnyy resurs] [Evaluation of the Emotional Stress of University Students [Electronic resource]. Materialy VIII Mezhdunarodnoy studencheskoy nauchnoy konferentsii «Studencheskiy nauchnyy forum» [Proceedings of the VIII International Student Scientific Conference "Student Scientific Forum"], URL : https://scienceforum.ru/2016/article/2016021979 (In Russ.)
8. Grinin, V. M., Avdeyev, D. A. (2012). Osobennosti okazaniya terapevticheskoy stomatologicheskoy pomoshchi letchikam voyennoy aviatsii v usloviyakh spetsializirovannogo LPU [Features of the provision of therapeutic dental care to pilots of military aviation in a specialized medical facility]. Rossiyskiy stomatologicheskiy zhurn [Russian dental journal], 3, 52-53. (In Russ.)
9. Zakharevich, N. V., Danilenko, V. N. (2017). Serin-treoninovyye proteinkinazy bakteriy - potentsial'naya mishen' dlya regulyatsii sostava mikrobioty cheloveka [Serine/threonine protein kinases of bacteria are potential targets for regulation of human microbiota composition]. Vestnik RGMU [Bulletin of Russian State Medical University], (2), 20-29. (In Russ.)
10. Makeeva, I. M., Bulgakov, V. S., Nikolskaia, I. A. (2008). Vliyaniye psikhoemotsional'nogo sostoyaniya patsiyenta na techeniye zabolevaniya parodonta [An influence of the psychoemotional condition on the course of periodontitis]. Zhurnal nauchnykh statey «Zdorov'ye i obrazovaniye v XXI veke» [Scientific magazine "A health and education in XXI century"], 1, 140-141. (In Russ.)
11. Averina, O. V., Danilenko, V. N. (2017). Mikrobiota kishechnika cheloveka: rol' v stanovlenii i funktsionirovanii nervnoy sistemy [Human intestinal microbiota: role in development and functioning of the nervous system]. Mikrobiologiya [Microbiology], 86, 1. (In Russ.)
12. D'yakovich, M. P., Semenikhin, V. A., Kazakova, P. V. (2014). Kachestvo zhizni lits s professional'noy patologiyey ot vozdeystviya razlichnykh proizvodstvennykh faktorov [The quality of life of persons with occupational pathology from the effects of various production factors]. Meditsina truda i promyshlennaya ekologiya [Occupational medicine and industrial ecology], 2, 27-32. (In Russ.)
13. Mikhailov, B. V., Serdiuk, A. I., Fedoseev, V. A. (2002). Psikhoterapiya v obshchesomaticheskoy meditsine: Klinicheskoye rukovodstvo [Psychotherapy in general medicine: Clinical tutorial]. Kharkov : Prapor. (In Russ.)
14. Pimenova, M. N., Grechushkina, N. N., Azova, L. G., Netrusova, A. I. (1995). Rukovodstvo k prakticheskim zanyatiyam po mikrobiologii : uchebnoye posobiye [A guide to practical lessons in microbiology. A tutorial]. Moscow : MSU-pub. (In Russ.)
15. Maksimovskiy, YU. M., Alekseyev, F. I., Martynova, S. A. (2009). Sostoyaniye tkaney parodonta u bol'nykh s narusheniyami psikhik [The state of periodontal tissues in patients with mental disorders]. Dental Forum [Dental Forum], 2 (30), 50-54. (In Russ.)
16. 16. Pryanichnikova, J. H. (2015). Ekzamenatsionnyy stress i yego profilaktika [Examination stress and its prevention]. Problemy i perspektivy razvitiya obrazovaniya: materialy VI Mezhdunar. nauch. konf. [Problems and prospects of educational development : materials of the VI International scientific conference], Perm : Mercure, 184-186. (In Russ.)
17. Sidorenko, E. V. (2015). Metody matematicheskoy obrabotki v psikhologii [Methods of mathematical processing in psychology]. St. Petersburg : Speech. (In Russ.)
18. Ichihashi, T., Nishinoue, N., Takada, K. (2015). Association between periodontal pockets and health-related behaviors of workers. Sangyo Eiseigaku Zasshi, 57 (1), 1-8.
19. Usmanova, I. N., Tuigunov, M. M., Gerasimova, L. P., Kabirova, M. F., Gubaidullin, A. G., Gerasimova, A. A., Husnarizanovna, R. F. (2015). Rol' uslovno-patogennoy mikroflory polosti rta v razvitii vospalitel'nykh zabolevaniy parodonta i slizistoy polosti rta (obzor literatury) [Role of opportunistic pathogenal microflora of the oral cavity in the development of inflammatory periodontal diseases and oral mucosa (literatura review)]. Chelovek. Sport. Meditsina [Human. Sport. Medicine], 2. (In Russ.)
20. Belova, A. N., Shchebetova, O. N. (2011). SHkaly, testy i oprosniki v medicinskoj reabilitacii: rukov.dlya vrachej i nauchn. sotr. [Scales, tests and questionnaires in medical rehabilitation: managers for doctors and scientific. sotr]. Moscow. (In Russ.)].
21. Metody vydeleniya chistoy kul'tury [Elektronnyy resurs] [Methods for the selection of pure culture [Electronic resource]. Studopediya [Studopedia], URL : http://studopedia.ru/7_23671_metodi-videleniya-chistoy-kulturi.html (In Russ.)
22. Tsepov, L. M., Nikolaev, A. I., Nesterova, M. M., Nakonechnyi, D. A. (2014). Problema etiologii vospalitel'nykh generalizovannykh zabolevaniy parodonta. Sovremennaya stomatologiya - effektivnost' profilaktiki i lecheniya. Nanotekhnologii v stomatologii [Problem of an etiology of inflammatory generalized diseases of the parodont. Modern stomatology - efficiency of prevention and treatment. Nanotechnologies in stomatology]. Materialy konferentsii, posvyashchennoy 60-letiyu TGMA [Materials of the conference dedicated to the 65th anniversary of the TverGMA], Tver, 310-319. (In Russ.)
23. Chereda, V. V., Petrushanko, T. A., Mamontova, T. V. (2014). Vliyaniye sezonnosti na sostoyaniye immunomikrobiotsinoza polosti rta u lits molodogo vozrasta [An influence of seasonality on the state of immunomicrobiocinosis of the oral cavityin young people]. Aktual'nyye problemy sovremennoy meditsiny: Vestnik Ukrainskoy meditsinskoy stomatologicheskoy akademii [Actual problems of the modern medicine. Bulletin of the Ukrainian Medical Dental Academy], 4 (48). (In Russ.)
24. De Iuliis, V., Ursi, S., Di Tommaso, L. M., Caruso, M., Marino, A., Ercole, S., Caputi, S., Sinjari, B., Festa, F., Macri, M., Martinotti, S., Vitullo, G., Toniato, E. J. (2016). Comparative molecular analysis of bacterial species associated with periodontal disease. Biol.Regul.Homeost. Agents, 30 (4), 1209-1215.
25. Razzouk, S. (2016). Regulatory elements and genetic variations in periodontal diseases. Arch Oral Biol, 72, 106-115. DOI:https://doi.org/10.1016/j.archoralbio.2016.08.015.
