MICROBIOLOGICAL ANALYSIS OF THE EFFECTIVENESS OF TABLETS FOR HYGIENIC TREATMENT OF REMOVABLE DENTURE SAMPLES (IN VITRO STUDY)
Abstract and keywords
Abstract:
Relevance. Removable dentures are widely used in orthopedic dentistry, however, the materials of their bases (PMMA) contribute to the adhesion of pathogenic and opportunistic microorganisms, followed by the formation of biofilms. This can cause dysbiotic changes in the microbiocenosis of the oral cavity, inflammatory reactions of the mucous membrane and the development of candidal stomatitis. In this regard, it is important to study the effectiveness of specialized means for hygienic treatment of prostheses and their effect on the residual adhesion of microorganisms. Materials and methods. In an in vitro experiment, PMMA samples were studied after treatment with six cleaning agents in tablet form. Reference strains of Escherichia coli ATCC 25982, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231, Streptococcus mutans ATCC 3003, S. mitis NCTC 10712, Porphyromonas gingivalis ATCC 33277 were used as test cultures. The index of residual adhesion was determined by the method of M.M. Davydov. Additionally, the effect of pretreatment with toothpaste was evaluated. Statistical analysis was performed using the Shapiro–Wilk and Mann–Whitney tests (p <0.05). Results. The control series revealed high rates of residual adhesion of all microorganisms. Splat Confident did not show a significant decrease in adhesion (p ≥0.05). PresiDent and Protefix provided a moderate effect. The most pronounced and statistically significant decrease in the residual adhesion index was found when using Corega Bio Formula and Corega Professional Max Clean (p <0.05). Pretreatment with toothpaste did not have a significant effect on adhesion. Conclusions. The effectiveness of cleaning agents for removable dentures varies significantly. The most effective are Corega Bio Formula and Corega Professional Max Clean. Regular use of specialized products is necessary to prevent microbial contamination and inflammatory complications.

Keywords:
removable dentures, hygiene products, residual adhesion, microbial biofilm, structural material
References

1. Kuzieva M.A. Kliniko-morfologicheskie kriterii organov rotovoy polosti pri primenenii nes'emnyh ortopedicheskih konstrukciy. Journal of Science in Medicine and Life. 2024;2(6):144-150. [Kuzieva M.A. Clinical and morphological criteria of the oral cavity organs in the use of non-removable orthopedic structures. Journal of Science in Medicine and Life. 2024;2(6):144-150. (In Russ.)]. https://journals.proindex.uz/index.php/JSML/article/view/1250

2. Rublenko S.S., Kungurov S.V., Osipova N.P., Kozlov V.V. Vliyanie s'emnyh ortopedicheskih konstrukciy na kolichestvennyy sostav mikroflory polosti rta. Sibirskoe medicinskoe obozrenie. 2010;(3):43–47. [Rublenko S.S., Kungurov S.V., Osipova N.P., Kozlov V.V. Influence of removable orthopedic constructions on composition of oral cavity microflora. Siberian Medical Review. 2010;(3):43–47 (In Russ.)]. https://elibrary.ru/item.asp?id=15126850

3. Rajendran A., Roshy G, Nicholas M., Ranjith M., Nazar N.A. Comparative evaluation of efficacy of three different denture cleansing methods in reducing Candida albicans count in removable partial denture wearers: A randomized controlled trial. Journal of Indian Prosthodontic Society. 2022;22(3):256-261. https://doi.org/10.4103/jips.jips_553_21

4. Aslanyan M.A. Profilaktika negativnogo vozdeystviya s'emnyh zubnyh protezov, proyavlyayuschegosya v vide allergicheskih reakciy na slizistoy obolochke proteznogo lozha. Byulleten' medicinskih internet-konferenciy. 2015;5(10):1183. [Aslanyan M.A. Prevention of the negative effects of removable dentures, manifested in the form of allergic reactions on the mucous membrane of the prosthetic bed. Bulletin of medical Internet conferences. 2015;5(10):1183. (In Russ.)]. https://elibrary.ru/item.asp?id=24931610

5. Sedghi L., DiMassa V., Harrington A., Lynch S.V., Kapila Y.L. The oral microbiome: Role of key organisms and complex networks in oral health and disease. Periodontology 2000. 2021;87(1):107-131. https://doi.org/10.1111/prd.12393

6. Axe A., Varghese R., Bosma M., Kitson N., Bradshaw D. Dental health professional recommendation and consumer habits in denture cleansing. The Journal Of Prosthetic Dentistry. 2016;115(2):183–188. https://doi.org/10.1016/j.prosdent.2015.08.007

7. Chirkova N.V., Morozov A.N., Vecherkina Zh.V., Pshenichnikov I.A., Popova T.A., Zayceva N.V. Sovremennye aspekty gigienicheskih meropriyatiy v ortopedicheskoy stomatologii. Voronezh: Voronezhskiy gosudarstvennyy medicinskiy universitet imeni N.N. Burdenko; 2019. 103 c. [Chirkova N.V., Morozov A.N., Vecherkina Zh.V., Pshenichnikov I.A., Popova T.A., Zaitseva N.V. Modern aspects of hygienic measures in prosthetic dentistry. Voronezh: N.N. Burdenko Voronezh State Medical University; 2019. 103 p. (In Russ.)]. https://elibrary.ru/item.asp?id=37302136&ysclid=mlkggr8pt428525878

8. Evavold C.L., Kagan J.C. How inflammasomes inform adaptive Immunity. Journal of molecular biology. 2018;430(2):217–237. https://doi.org/10.1016/j.jmb.2017.09.019

9. Chesnokova M.G., Chesnokov V.A., Mironov A.Yu. Vidovoy spektr mikrobioma poverhnosti s'emnyh zubnyh protezov pacientov na etapah provedeniya ortopedicheskoy reabilitacii. Uspehi medicinskoy mikologii 2023;24:174-177. [Chesnokova M.G., Chesnokov V.A., Mironov A.Yu. Species spectrum of the microbiome of the surface of removable dentures of patients at the stages of orthopedic rehabilitation. Uspehi medicinskoj mikologii. 2023;24:174-177. (In Russ.)]. https://elibrary.ru/apvott?ysclid=mlkg8m87t299677517

10. Chesnokova M.G., Chesnokov V.A., Zherebcov V.V., Mironov A.Yu. Analiz sostoyaniya slizistoy obolochki polosti rta pacientov s kandidoznym stomatitom i mikrobioma poverhnosti s'emnyh zubnyh protezov pri ortopedicheskoy reabilitacii. Klinicheskaya laboratornaya diagnostika. 2024;69(12):693-699. [Chesnokova M.G., Chesnokov V.A., Zherebtsov V.V., Mironov A.Yu. Analysis of the state of the oral mucosa of patients with candidal stomatitis and the microbiome of the surface of removable dentures during orthopedic rehabilitation. Clinical laboratory diagnostics. 2024;69(12):693-699. (In Russ.)]. https://doi.org/10.51620/0869-2084-2024-69-12-693-699

11. Vecherkina Zh.V., Chirkova N.V., Andreeva E.A., Dronov D.V., Gurov D.V. Sovershenstvovanie profilakticheskogo obespecheniya pri ortopedicheskom lechenii s'emnymi zubnymi protezami. Sistemnyy analiz i upravlenie v biomedicinskih sistemah. 2022;21(1):16-20. [Vecherkina Zh.V., Chirkova N.V., Andreeva E.A., Dronov D.V., Gurov D.V. Improvement of preventive maintenance in orthopedic treatment with removable dentures. Sistemnyj analiz i upravlenie v biomedicinskih sistemah. 2022;21(1):16-20. (In Russ.)]. https://doi.org/10.36622/VSTU.2022.21.1.002

12. Carev V.N., Stepanov A.G., Ippolitov E.V., Podporin M.S., Careva T.V. Kontrol' pervichnoy adgezii mikroorganizmov i formirovaniya bioplenok na stomatologicheskih materialah, ispol'zuemyh dlya transdental'noy implantacii pri zubosohranyayuschih operaciyah. Klinicheskaya laboratornaya diagnostika. 2018;63(9):568-573. [Tsarev V.N., Stepanov A.G., Ippolitov E.V., Podporin M.S., Tsareva T.V. Control of primary adhesion of microorganisms and formation of biofilms on stomatological materials used for transdental implantation in dental stabilizing operations. Clinical laboratory diagnostics. 2018;63(9):568-573. (In Russ.)]. https://clinlabdia.ru/article/kontrol-pervichnoj-adgezii-mikroorg/


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