Subject. The health status of children and adolescents is one of the most acute medical and social problems. It is known, that with dental diseases, changes occur not only in the immunological profile of the oral fluid, but also in morphology of the oral tissues. New approaches to the traditional cytological study of buccal epithelium such as analysis of the cytogram with the isolation of various types of cells, as well as the detection of cytological abnormalities of cells, allows us to evaluate the reactivity of the oral mucosa in pathological processes. According to WHO recommendations (2013), groups of children 5-6, 12, 15 years of age are the global indicator age groups for monitoring disease trends and comparisons on an international scale. The objective of the study is to assess the health status of children age 5-6, 12, 15 with non-invasive methods. It is based on the results of a clinical and laboratory examination of 179 children, attending organized children's groups. Children underwent a comprehensive dental examination, which included a questionnaire according to the WHO method, an external examination of the maxillofacial region, an examination of the oral cavity, identification of pathology of hard tooth tissues. Methodology. We studied the change in the dental status of patients, indicators of oral fluid and basal epithelium with age, in order to prognostically use non-invasive assessment methods in a comprehensive health examination, planning and evaluating the effectiveness of prevention programs. Results. It was noted that the dental health status of children 5-6, 12, 15 years old can be assessed as satisfactory, while dental, laboratory and cytological health indicators worsen with age. Non-invasive methods for assessing the dental status of patients can be used in a comprehensive examination of children's health, planning and evaluating the effectiveness of prevention programs.